中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2011年
9期
40-43
,共4页
刘正才%杨方%余孔捷%张琼%林永辉%陈瑞清
劉正纔%楊方%餘孔捷%張瓊%林永輝%陳瑞清
류정재%양방%여공첩%장경%림영휘%진서청
液相色谱-串联质谱法%兽药粉剂%氯霉素类%硝基呋喃类
液相色譜-串聯質譜法%獸藥粉劑%氯黴素類%硝基呋喃類
액상색보-천련질보법%수약분제%록매소류%초기부남류
liquid chromatography - tandem mass spectrometry%powder of veterinary drug preparation%chloramphenicols%nitrofurans
建立了兽药粉剂中氯霉素类和硝基呋喃类共7种抗菌药物的液相色谱-串联质谱筛选方法。样品经水溶解,乙酸乙酯提取样品中的药物,OasisHLB固相萃取小柱净化,液相色谱-串联质谱仪测定。分别对该方法的检测限和专属性进行了实验室内和实验室外验证。结果表明,该方法简单、灵敏、特异性强,适用于兽药粉剂中氯霉素类和硝基呋喃类的定性筛选测定。
建立瞭獸藥粉劑中氯黴素類和硝基呋喃類共7種抗菌藥物的液相色譜-串聯質譜篩選方法。樣品經水溶解,乙痠乙酯提取樣品中的藥物,OasisHLB固相萃取小柱淨化,液相色譜-串聯質譜儀測定。分彆對該方法的檢測限和專屬性進行瞭實驗室內和實驗室外驗證。結果錶明,該方法簡單、靈敏、特異性彊,適用于獸藥粉劑中氯黴素類和硝基呋喃類的定性篩選測定。
건립료수약분제중록매소류화초기부남류공7충항균약물적액상색보-천련질보사선방법。양품경수용해,을산을지제취양품중적약물,OasisHLB고상췌취소주정화,액상색보-천련질보의측정。분별대해방법적검측한화전속성진행료실험실내화실험실외험증。결과표명,해방법간단、령민、특이성강,괄용우수약분제중록매소류화초기부남류적정성사선측정。
A method based on liquid chromatographytandem mass spectrometry (HPLC -MS/MS) was developed for the screening of about 7 kinds of chloramphenicols and nitrofurans in powder of veterinary drug preparation. The analyte was dissolved by water, extracted with ethyl acetate, and cleaned by Oasis HLB solid phase extraction cartridge. Qualitative analysis of the analyte was achieved by HPLC - MS/MS with multiple reaction monitoring (MRM). The limit of detection and specificity of method was validated through within laboratory test and inter laboratory test respectively. The results showed that the established method was fast, sensitive, specific and quite suitable for identification and qualitative analysis of chloramphenicols and nitrofurans in powder of veterinary drug preparation.