CAJ | 학술논문

建立一种新型藏羊血红素的提取方法。以藏羊血为原料,丙酮和酸性丙酮为提取溶剂,所得粗提物进行纯化后,采用红外光谱法对血红素进行定性分析,并用紫外分光光度法进行定量测定。通过进行单因素试验,可确定丙酮添加量为血液体积1倍、离心时间为15min是制备藏羊血红素的最优条件,该法制备血红素成本低,操作过程简便,生产周期短,纯度可达98.60%,且收率达到（87.91±9.04）%。
건립일충신형장양혈홍소적제취방법。이장양혈위원료,병동화산성병동위제취용제,소득조제물진행순화후,채용홍외광보법대혈홍소진행정성분석,병용자외분광광도법진행정량측정。통과진행단인소시험,가학정병동첨가량위혈액체적1배、리심시간위15min시제비장양혈홍소적최우조건,해법제비혈홍소성본저,조작과정간편,생산주기단,순도가체98.60%,차수솔체도（87.91±9.04）%。
To establish a new extract method of Tibetan sheep heme.The crude extracts of Tibetan sheep blood as the raw material,acetone and acid acetone as solvent,was purified.Infrared spectroscopy was applied for qualitative analysis of heme,and ultraviolet spectrophotometry was utilized for quantitative determination of heme.By single-factor test to determine that the dosage of acetone is blood volume of 1 times and centrifugation time is 15min,which is the optimal preparation conditions of Tibetan sheep heme.The method of preparation of heme makes the low-cost,simple operation,short production cycle,purity and yield can reach respectively 98.60% and（87.91±9.04）%.