CAJ | 학술논문

目的：获得一种廉价的、核酸酶（RNase）处理效果好的载玻片运用于原位杂交技术中。方法：改进传统APES（3-Aminopropyl triethoxysilane）方法处理载玻片,加入载玻片高温烘烤、丙酮稀释APES等步骤,以去除RNase污染,并将处理好的载玻片分别进行冰冻和石蜡组织贴片,用原位杂交试验进行验证。结果和结论：原位杂交结果显示两种组织贴片效果良好,组织未出现掉片,RNA信号特异,与进口载玻片没有差异。因此,改进APES方法处理的载玻片完全能够运用于原位杂交技术中,可大大节约试验成本。
목적：획득일충렴개적、핵산매（RNase）처리효과호적재파편운용우원위잡교기술중。방법：개진전통APES（3-Aminopropyl triethoxysilane）방법처리재파편,가입재파편고온홍고、병동희석APES등보취,이거제RNase오염,병장처리호적재파편분별진행빙동화석사조직첩편,용원위잡교시험진행험증。결과화결론：원위잡교결과현시량충조직첩편효과량호,조직미출현도편,RNA신호특이,여진구재파편몰유차이。인차,개진APES방법처리적재파편완전능구운용우원위잡교기술중,가대대절약시험성본。
Objective：To obtain a kind of cheap glass slides without RNase pollution,then could be used to in situ hybridization.Method：An improved APES（3-Aminopropyl triethoxysilane） treatment method was used to remove RNase pollution through baking glass slides at high temperature and diluting APES with acetone.These glass slides were used to stick the paraffin and frozen tissues,then the experimental effects of these glass slides were evaluated by in situ hybridization.Results and Conclusion：Both paraffin and frozen sections were well fit for in situ hybridization without any ecclasis of tissues,and specific RNA signals were detected.Our findings indicated that the effects of these APES-treated glass slides could be equal to the imported ones.Therefore,we concluded these APES-treated glass slides can absolutely be used to in situ hybridization,moreover,the experimental cost will be saved remarkably.