草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2011年
6期
18-26
,共9页
赵海明%孙桂枝%王学敏%刘贵波
趙海明%孫桂枝%王學敏%劉貴波
조해명%손계지%왕학민%류귀파
百脉根%苗期%反复干旱法%抗旱性%综合评价
百脈根%苗期%反複榦旱法%抗旱性%綜閤評價
백맥근%묘기%반복간한법%항한성%종합평개
Lotus corniculatus%seedling stage%repeated drought method%drought resistance%comperehensive evaluation
采用土培法对21份百脉根种质材料进行了苗期抗旱性评价。结果表明:在干旱胁迫下,明显抑制百脉根的正常生长,地上生物量、地下生物量、株高、分枝数明显减小,根冠比明显增加;渗透调节物质丙二醛(MDA)、脯氨酸(PR0)、可溶性糖、可溶性蛋白的含量明显增加,过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性比正常灌水处理明显增强;运用了6种方法进行抗旱性评价,其中,五级评分法和标准差系数赋予权重法的准确度较高,聚类分析是较好的分类法,二者相结合效果更好;依据标准差系数赋予权重法与聚类分析结果,可将百脉根种质分为3类:抗旱性强的有B90、B85、B78、B79、B83、B92、B81,抗旱性中等的有B73、B89、B76、B93、B75、B86、B91,抗旱性弱的有B77、B84、B74、B80、B82、B88、B87。
採用土培法對21份百脈根種質材料進行瞭苗期抗旱性評價。結果錶明:在榦旱脅迫下,明顯抑製百脈根的正常生長,地上生物量、地下生物量、株高、分枝數明顯減小,根冠比明顯增加;滲透調節物質丙二醛(MDA)、脯氨痠(PR0)、可溶性糖、可溶性蛋白的含量明顯增加,過氧化物酶(POD)、過氧化氫酶(CAT)、超氧化物歧化酶(SOD)活性比正常灌水處理明顯增彊;運用瞭6種方法進行抗旱性評價,其中,五級評分法和標準差繫數賦予權重法的準確度較高,聚類分析是較好的分類法,二者相結閤效果更好;依據標準差繫數賦予權重法與聚類分析結果,可將百脈根種質分為3類:抗旱性彊的有B90、B85、B78、B79、B83、B92、B81,抗旱性中等的有B73、B89、B76、B93、B75、B86、B91,抗旱性弱的有B77、B84、B74、B80、B82、B88、B87。
채용토배법대21빈백맥근충질재료진행료묘기항한성평개。결과표명:재간한협박하,명현억제백맥근적정상생장,지상생물량、지하생물량、주고、분지수명현감소,근관비명현증가;삼투조절물질병이철(MDA)、포안산(PR0)、가용성당、가용성단백적함량명현증가,과양화물매(POD)、과양화경매(CAT)、초양화물기화매(SOD)활성비정상관수처리명현증강;운용료6충방법진행항한성평개,기중,오급평분법화표준차계수부여권중법적준학도교고,취류분석시교호적분류법,이자상결합효과경호;의거표준차계수부여권중법여취류분석결과,가장백맥근충질분위3류:항한성강적유B90、B85、B78、B79、B83、B92、B81,항한성중등적유B73、B89、B76、B93、B75、B86、B91,항한성약적유B77、B84、B74、B80、B82、B88、B87。
The drought resistance of 21 accessions of Lotus corniculatus germplasms materials were evalua ted in seedling stage. The results indicated that the normal growth in seedling period was significantly inhibited under drought stress, the aboveground biomass, underground biomass, plant height and branch number decreased significantly, root-shoot ratio increased; Malondialdehyde (MDA), proline (PRO), soluble sugar and soluble protein contents increased significantly under the drought stress;Activity of peroxidase(POD), catalase(CAT) and superoxide dismutase (SOD) were increased more than that under the drought stress;6 methods were used for the comprehensive evaluation of the drought resistance, the five level grade methods and the standard deviation coefficient allocation weighted method showed the good evaluation results. 21 accessions of L. corniculatus germplasm were classified into 3 groups by clustering analysis and the standard deviation coefficient allocation weighted method. The germplasms with stronger drought resistance were Bg0, B85, B78, B79, B83, B92 and B81, while B73,B89, B76, B93 ,B75,B86 and B91 were the middle,B77,B84,B74,B80,B82,B88 and B87 were the lowest.