CAJ | 학술논문

生肌调节因子（MRF）基因家族的表达决定着肌细胞的命运，并控制其增殖和分化．Myf5是生肌调节因子MRF基因家族中的一个成员．本文提取赤眼鳟肌肉总RNA，采用RT—PCR技术获得赤眼鳟Myf5基因的部分cDNA序列，共338bp，编码112个氨基酸，含有部分bHLH结构．通过同源性分析发现赤眼鳟Myf5基因编码区与草鱼的同源性高达97．34％．半定量RT—PCR分析表明赤眼鳟Myf5基因在心脏和肾脏中表达量较高，鳃和肌肉中表达量次之，肝脏、脑和肠中表达量较弱．本结果为进一步研究该基因对肌肉生长发育的作用机理奠定了基础．
생기조절인자（MRF）기인가족적표체결정착기세포적명운，병공제기증식화분화．Myf5시생기조절인자MRF기인가족중적일개성원．본문제취적안준기육총RNA，채용RT—PCR기술획득적안준Myf5기인적부분cDNA서렬，공338bp，편마112개안기산，함유부분bHLH결구．통과동원성분석발현적안준Myf5기인편마구여초어적동원성고체97．34％．반정량RT—PCR분석표명적안준Myf5기인재심장화신장중표체량교고，새화기육중표체량차지，간장、뇌화장중표체량교약．본결과위진일보연구해기인대기육생장발육적작용궤리전정료기출．
The expression of the myogenic regulation factors （MRF） family determines the fate of myocytes, and controls its proliferation and differentiation. Myf5 is a member of MRF family. Total RNA of Squaliobarbus curriculus was extracted from muscle tissues. A 338bp long fragment of Myf5 cDNA in Squaliobarbus curriculus was obtained by using RT-PCR, which encodes 112 aa peptide containing partial HLH helix domain. Compared with other species, Squaliobarbus curriculus has the highest homology with Ctenopharyngodon idella. The results of tis- sue expression detected by semi-quantitative RT-PCR showed that myf5 gene was widely expressed in various tissues of Squaliobarbus curriculus, expressed much higher in kidney and heart than in gill and muscle, while it was weakly expressed in liver, brain and intestine.