湖北民族学院学报：自然科学版
湖北民族學院學報：自然科學版
호북민족학원학보：자연과학판
Journal of Hubei Institute for Nationalities(Natural Sciences)
2011年
3期
295-298
,共4页
pH值%根霉%产酶条件%酶活性
pH值%根黴%產酶條件%酶活性
pH치%근매%산매조건%매활성
pH value%Rhizopus%enzyme-producing requirements%enzyme activeness level
在不同的pH值培养基中培养ERh1123、ERh3421、ERh5131 3个根霉菌株,研究其产酶的pH条件,同时将得到的酶液在不同的pH值条件下与一定的底物反应,分别测定了糖化酶、液化酶、蛋白酶活性,研究了pH条件对产酶活性的影响。结果表明：菌株产糖化酶、液化酶的适宜pH为4.6～5.2,在此条件下,糖化酶的活性为1.41×102～1.59×102U/g,液化酶的活性为0.69×103～2.21×103U/g,菌株产蛋白酶的适宜pH为5.8,其活性为1.98×103U/g～2.58×102U/g;反应时糖化酶适宜的pH为4.6,蛋白酶适宜的pH为5.8,液化酶适宜的pH为5.2-5.8,反应时酶的活性与产酶条件下相比,前两种酶的活性可分别提高到1.52×102～3.21×102U/g,2.56×102～2.97×102U/g,但液化酶的活性没有增加.总体来看,在根霉菌株产酶及酶反应时提供适宜的pH值条件对酶活性的提高具有很大的促进作用.
在不同的pH值培養基中培養ERh1123、ERh3421、ERh5131 3箇根黴菌株,研究其產酶的pH條件,同時將得到的酶液在不同的pH值條件下與一定的底物反應,分彆測定瞭糖化酶、液化酶、蛋白酶活性,研究瞭pH條件對產酶活性的影響。結果錶明：菌株產糖化酶、液化酶的適宜pH為4.6～5.2,在此條件下,糖化酶的活性為1.41×102～1.59×102U/g,液化酶的活性為0.69×103～2.21×103U/g,菌株產蛋白酶的適宜pH為5.8,其活性為1.98×103U/g～2.58×102U/g;反應時糖化酶適宜的pH為4.6,蛋白酶適宜的pH為5.8,液化酶適宜的pH為5.2-5.8,反應時酶的活性與產酶條件下相比,前兩種酶的活性可分彆提高到1.52×102～3.21×102U/g,2.56×102～2.97×102U/g,但液化酶的活性沒有增加.總體來看,在根黴菌株產酶及酶反應時提供適宜的pH值條件對酶活性的提高具有很大的促進作用.
재불동적pH치배양기중배양ERh1123、ERh3421、ERh5131 3개근매균주,연구기산매적pH조건,동시장득도적매액재불동적pH치조건하여일정적저물반응,분별측정료당화매、액화매、단백매활성,연구료pH조건대산매활성적영향。결과표명：균주산당화매、액화매적괄의pH위4.6～5.2,재차조건하,당화매적활성위1.41×102～1.59×102U/g,액화매적활성위0.69×103～2.21×103U/g,균주산단백매적괄의pH위5.8,기활성위1.98×103U/g～2.58×102U/g;반응시당화매괄의적pH위4.6,단백매괄의적pH위5.8,액화매괄의적pH위5.2-5.8,반응시매적활성여산매조건하상비,전량충매적활성가분별제고도1.52×102～3.21×102U/g,2.56×102～2.97×102U/g,단액화매적활성몰유증가.총체래간,재근매균주산매급매반응시제공괄의적pH치조건대매활성적제고구유흔대적촉진작용.
The pH reaction conditions for enzyme-producing of three Rhizopus of ERh1123,ERh3421 and ERh5131,which was cultivated in culture medium with different pH value,and the activeness level of saccharified enzyme,liquefied enzyme and protease achived from aboved culture medium have been respectively measured in reactions with certain substrates,and analysed the influence of enzyme-producing by pH value.The study shows that,at optimal culture medium of pH value of 4.6～5.2,the activeness of saccharified enzymes produced by above strains can be reached at level of 1.41×102～1.59×102U/g,and liquefied enzymes 0.69×103～2.21×103U/g;and for protease the activeness can reach 1.98×103U/g ～2.58×102 U/g in culture medium with 5.8 of pH value;in reaction with certain substrates,the optimal level of pH value for saccharified enzyme was 4.6,protease 5.8,liquefied enzyme 5.2～5.8,and the activeness of enzyme of the former two,in contrast to that at condition of enzyme-producing,can be respectively improved to 1.52×102～3.21×102U/g and 2.56×102～2.97×102U/g,but no change for liquefied enzyme.Generally,there exists considerable improving for enzyme activeness levels by pH value of culture medium and of reaction liquied with substrates.
