CAJ | 학술논문

目的观察不同剂量氯胺酮对突触快速生长期大鼠神经元的损伤作用。方法 0.01、0.1、1和2mmol/L氯胺酮（K1、K2、K3和K4组）及单纯培养基（C组）作用于体外原代培养第6天的大鼠神经元24h。药物洗脱24h后,通过MTT细胞还原检测、原位荧光TUNEL染色、LDH释放检测及电镜观察神经元损伤程度。结果与C组相比,K3和K4组神经元MTT显著降低（P〈0.05）,TUNEL阳性细胞百分比显著增高（P〈0.05）,电镜检查显示凋亡细胞增多。结论大剂量氯胺酮可致体外原代培养大鼠皮层神经元凋亡。
목적관찰불동제량록알동대돌촉쾌속생장기대서신경원적손상작용。방법 0.01、0.1、1화2mmol/L록알동（K1、K2、K3화K4조）급단순배양기（C조）작용우체외원대배양제6천적대서신경원24h。약물세탈24h후,통과MTT세포환원검측、원위형광TUNEL염색、LDH석방검측급전경관찰신경원손상정도。결과여C조상비,K3화K4조신경원MTT현저강저（P〈0.05）,TUNEL양성세포백분비현저증고（P〈0.05）,전경검사현시조망세포증다。결론대제량록알동가치체외원대배양대서피층신경원조망。
Objective To observe the effect of different doses of ketamine on rat neuronal injury during the period of rapid synaptogenesis. Methods Ketamine 0.01,0.1,1,and 2 mmol/L （K1,K2,K3,and K4 groups） or cell culture media （C group） was applied to primary rat neuronal cultures at development in vitro 6 d for 24 h. Twenty four hours after ketamine washout,neuron injury was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide （MTT） cell reduction assay,in situ terminal deoxynucleotidyl transferase （TdT）-mediated fluorescein-dUTP nick end labeling terminal （TUNEL） staining,cytosolic lactate dehydrogenase （LDH） release detection,and electron microscopic examination.Results Compared with C group,neurons in K3 or K4 group showed a decreased MTT value （P0.05） and increased percentage of TUNEL-positive cells （P0.05）. Furthermore,electron micrographs showed more apoptotic neurons in K3 or K4 group. However,no significant difference was observed in the release of LDH in different groups.Conclusion Large dose of ketamine may induce apoptosis in primary rat neuronal cultures.