CAJ | 학술논문

[目的]研究高氟低碘对大鼠肝脏细胞DNA损伤的影响.[方法]离乳Wistar大鼠21只,随机分为4组:①CK(5只),饲喂大鼠标准饲料,饮自来水;②高氟组(5只),饲喂大鼠正常饲料,饮100 mg/L氟化钠(NaF)去离子水;③低碘组(5只),饲喂低碘饲料(定做),饮去离子水;④高氟低碘组(6只),饲喂低碘饲料,饮100 mg/L氟化钠(NaF)去离子水.在大鼠20月龄时,处死取肝脏组织,检查其DNA的损伤情况.[结果]与①CK相比,②、③和④的老年大鼠肝细胞拖尾率显著增高(P＜0.01);②、③和④的老年大鼠肝细胞的拖尾长度亦显著增长(P＜0.01);肝细胞DNA损伤分级结果显示,②(高氟组)老年大鼠肝细胞DNA损伤主要是Ⅱ级、Ⅲ级.③(低碘组)肝细胞DNA几乎全部损伤,主要是Ⅲ级损伤.④(高氟低碘组)的细胞损伤以Ⅲ级损伤为主.[结论]高氟、低碘、高氟低碘都影响了大鼠肝脏细胞DNA 的损伤.
[목적]연구고불저전대대서간장세포DNA손상적영향.[방법]리유Wistar대서21지,수궤분위4조:①CK(5지),사위대서표준사료,음자래수;②고불조(5지),사위대서정상사료,음100 mg/L불화납(NaF)거리자수;③저전조(5지),사위저전사료(정주),음거리자수;④고불저전조(6지),사위저전사료,음100 mg/L불화납(NaF)거리자수.재대서20월령시,처사취간장조직,검사기DNA적손상정황.[결과]여①CK상비,②、③화④적노년대서간세포타미솔현저증고(P＜0.01);②、③화④적노년대서간세포적타미장도역현저증장(P＜0.01);간세포DNA손상분급결과현시,②(고불조)노년대서간세포DNA손상주요시Ⅱ급、Ⅲ급.③(저전조)간세포DNA궤호전부손상,주요시Ⅲ급손상.④(고불저전조)적세포손상이Ⅲ급손상위주.[결론]고불、저전、고불저전도영향료대서간장세포DNA 적손상.
[Objective] The research aimed to study the effects of high-fluoirde and low-iodine on DNA damage of liver cells in rats. [Method] 21 weaned Wistar rats were randomly divided into 4 groups, including ① CK (feeding 5 rats with standard diet and tap water), ② high-F group (feeding 5 rats with normal diet and 100 mg/L NaF deionized water), ③ low-I group (feeding 5 rats with low-I diet and deionized water) and ④ high-F and low-I group (feeding 6 rats with low-I diet and 100 mg/L NaF deionized water). On the 20th month, rats were killed and their liver tissues were taken to detect the situations of DNA damage. [Result] Compared with CK, the tailing rate of liver cells in aged rats in groups ②, ③ and ④ was significantly increased (P＜0.01) and the tail length of liver cells in aged rats in groups ②, ③ and ④ was longer(P＜0.01). The classification results of DNA damage in liver cells indicated that DNA damage in liver cells in aged rats in group ② were mainly Ⅱ-class and Ⅲ -class. DNA in liver cells in group ③ were almost damaged, being mainly Ⅲ-class. The cell damage in group ④ was Ⅲ -class. [Conclusion] High-F, low-I, high-F and low-I all affected DNA damage in liver cells in rats.