CAJ | 학술논문

Bcl-2家族蛋白是目前已知的细胞凋亡中最重要的调控因子,在细胞凋亡通路中起着重要的调节作用.本研究通过把外源bcl-2基因转入栗疫病菌中,采用RT-PCR的方法检测bcl-2基因在栗疫病菌株中的表达情况,对表达bcl-2基因的转化子及野生型的菌株进行病毒传递率和致病性测定.结果表明:表达bcl-2基因的转化子表现出病毒传递率提高、致病性增强等一系列特征.由此可知:bcl-2基因对栗疫病菌细胞凋亡起调节作用,并对病菌的生物学特征有一定影响.
Bcl-2가족단백시목전이지적세포조망중최중요적조공인자,재세포조망통로중기착중요적조절작용.본연구통과파외원bcl-2기인전입률역병균중,채용RT-PCR적방법검측bcl-2기인재률역병균주중적표체정황,대표체bcl-2기인적전화자급야생형적균주진행병독전체솔화치병성측정.결과표명:표체bcl-2기인적전화자표현출병독전체솔제고、치병성증강등일계렬특정.유차가지:bcl-2기인대률역병균세포조망기조절작용,병대병균적생물학특정유일정영향.
Proteins belonging to the Bcl-2 family are key regulators of apoptosis. Here, bcl-2 gene was transformed into protoplast of Cryphonectria parasitwa and the expression of bcl-2 gene was tested. Further, hypovirus transportation rate of transformants and their hyper virulent to host were also tested. The results indicated that strains expressing the anti-apoptotic Bcl-2 protein could enhance hypovirus transmission rate and were hyper virulent to host. Thus bcl-2 gene also played a regulatory role in apoptosis of C. parasitica and had some infection in the biological characters.