CAJ | 학술논문

新西兰兔骨髓基质成骨细胞作为接种珊瑚的种子细胞。珊瑚块分别用多聚赖氨酸、纤维粘连蛋白（Fn）和单纯培养液处理，接种成骨细胞后形成细胞/珊瑚复合物，在体外培养7、14、21天进行观察。电镜显示，第7天和14天，Fn处理的珊瑚表面有更多的成骨细胞粘附和骨基质的形成。经多聚赖氨酸处理的珊瑚与培养液处理的珊瑚比较，虽然在第7天通过DNA含量的测定具有较多的细胞数，但在第14、21天差异无显著性意义。Fn处理的珊瑚在各时间点细胞数和碱性磷酸酶活性均高于多聚赖氨酸和培养液处理组，同时细胞数随培养时间呈增长趋势。本实验结果提示Fn处理珊瑚表面可以明显促进成骨细胞的粘附和增殖。
신서란토골수기질성골세포작위접충산호적충자세포。산호괴분별용다취뢰안산、섬유점련단백（Fn）화단순배양액처리，접충성골세포후형성세포/산호복합물，재체외배양7、14、21천진행관찰。전경현시，제7천화14천，Fn처리적산호표면유경다적성골세포점부화골기질적형성。경다취뢰안산처리적산호여배양액처리적산호비교，수연재제7천통과DNA함량적측정구유교다적세포수，단재제14、21천차이무현저성의의。Fn처리적산호재각시간점세포수화감성린산매활성균고우다취뢰안산화배양액처리조，동시세포수수배양시간정증장추세。본실험결과제시Fn처리산호표면가이명현촉진성골세포적점부화증식。
The marrow stromal osteoblasts from New Zealand rabbits were seeded on coral treated with L-polylysine, fibronectin and culture medium only, then the cells/coral composites were cultured in vitro. The cells/coral composites were observed for the process of cells growth and matrix formation at 7, 14 and 21 days after culture. With the aid of electron microscope,it was demonstrated that on the surface of the coral holes,which was treated with fibronectin, there were more adhering osteoblasts and matrix formation than those treated with both L-polylysine and culture medium after 7 and 14 days.Cell count in coral blocks was determined by doing a fluorimetric DNA assay. Although the samples treated with L-polylysine demonstrated higher cell count than the coral treated with culture medium after 7 days, there was no statistically difference between the two after 14, 21 days. At each time point, the samples treated with fibronectin showed higher cell count and alkaline phosphatase activity than the orals otherwise treated, and the cell count also increased with culture time. The study suggests that fibronectin has a significant effect on promoting the adhesion and proliferation of osteoblasts on the surface of coral.