CAJ | 학술논문

目的探讨重复性短时间间隔皮下注射镉金属硫蛋白(CdMT)对实验小鼠肾脏损伤的时程变化。方法染毒组小鼠给予首次高剂量(0.05mg/kg)和随后重复4次相同低剂量(0.025mg/kg)的CdMT，每次间隔时间为2h,在首次染毒后12、24、72、168和336h处死动物，对照组注射相同体积的生理盐水。观察肝、肾皮质组织中镉、钙以及尿液中镉、钙及尿蛋白的变化情况。结果皮下注射CdMT后，肾皮质中钙含量均高于对照组，在染CdMT后第24及336h肾皮质钙含量明显升高，且随着时间的延长尿蛋白、尿钙也有升高的趋势，尿蛋白、尿钙的高峰均出现在注射CdMT后168h。结论重复性短时间间隔皮下注射CdMT造成小鼠肾脏损害可维持较长的时间，钙稳态失衡是可能的毒作用机制之一。
목적탐토중복성단시간간격피하주사력금속류단백(CdMT)대실험소서신장손상적시정변화。방법염독조소서급여수차고제량(0.05mg/kg)화수후중복4차상동저제량(0.025mg/kg)적CdMT，매차간격시간위2h,재수차염독후12、24、72、168화336h처사동물，대조조주사상동체적적생리염수。관찰간、신피질조직중력、개이급뇨액중력、개급뇨단백적변화정황。결과피하주사CdMT후，신피질중개함량균고우대조조，재염CdMT후제24급336h신피질개함량명현승고，차수착시간적연장뇨단백、뇨개야유승고적추세，뇨단백、뇨개적고봉균출현재주사CdMT후168h。결론중복성단시간간격피하주사CdMT조성소서신장손해가유지교장적시간，개은태실형시가능적독작용궤제지일。
Objective To explore the time course of the renal impacts inducedby multiple short-interval injections of cadmium-metallothionein (CdMT) in female mice.Methods The mice were given with an initial high dose and with four subsequent low doses (0.05＋4×0.025 mg/kg) subcutaneously at 2 h intervals. The excretion of cadmium, calcium and protein in urine and changes of calcium and level in kidney cortex were studied following the first injection. Results The calcium level in kidney cortex were significantly higher at 24 and 336 h after the first injection. The excretion of calcium and protein in urine appeared to be markedly increased to a maxiumal value at 168 h and kept until 336 h. Conclusion Marked nephrotoxic effects after multiple short-interval CdMT injections were shown with rather long duration. The disturbance of calcium homeostasis might be one of the possible mechanisms of such renal impacts.