中国实用妇科与产科杂志
中國實用婦科與產科雜誌
중국실용부과여산과잡지
CHINESE JOURNAL OF PRACTICAL GYNECOLOGY AND OBSTETRICS
2001年
3期
149-151
,共3页
梁劲荃%赵或明%梁睿%主改霞%孙润琴
樑勁荃%趙或明%樑睿%主改霞%孫潤琴
량경전%조혹명%량예%주개하%손윤금
滋养细胞肿瘤葡萄胎免疫组织化学p16CyclinD1
滋養細胞腫瘤葡萄胎免疫組織化學p16CyclinD1
자양세포종류포도태면역조직화학p16CyclinD1
目的探讨p16、CyclinD1基因在滋养细胞肿瘤发生方面的意义。方法用免疫组化SP法,对49例滋养细胞疾病组织(葡萄胎首次清宫标本32例,侵蚀性葡萄胎及绒癌子宫标本分别为12例和5例)和4例早孕正常绒毛组织中p16和CyclinD1进行检测。结果在早孕绒毛组织中,pi6和CyclinD1表达分别为全部阳性和全部阴性。在不恶变葡萄胎、以后发生恶变的葡萄胎和滋养细胞肿瘤中p16表达呈下降趋势,CyclinD1表达呈上升趋势。p16和CyclinD1表达阳性率在不恶变葡萄胎与以后发生恶变的葡萄胎组间、在不恶变葡萄胎组与侵蚀性葡萄胎间均有显著差异(P<0.05);而以后发生恶变的葡萄胎与侵蚀性葡萄胎间及侵蚀性葡萄胎与绒毛膜癌间无显著差异(P>0.05)。结论 p16表达缺失、CyclinD1过表达可能在滋养细胞恶性转化、侵蚀性葡萄胎发生中起重要作用。检测葡萄胎首次清官标本p16及CyclinD1表达,可能在判断葡萄胎预后方面具有重要意义。
目的探討p16、CyclinD1基因在滋養細胞腫瘤髮生方麵的意義。方法用免疫組化SP法,對49例滋養細胞疾病組織(葡萄胎首次清宮標本32例,侵蝕性葡萄胎及絨癌子宮標本分彆為12例和5例)和4例早孕正常絨毛組織中p16和CyclinD1進行檢測。結果在早孕絨毛組織中,pi6和CyclinD1錶達分彆為全部暘性和全部陰性。在不噁變葡萄胎、以後髮生噁變的葡萄胎和滋養細胞腫瘤中p16錶達呈下降趨勢,CyclinD1錶達呈上升趨勢。p16和CyclinD1錶達暘性率在不噁變葡萄胎與以後髮生噁變的葡萄胎組間、在不噁變葡萄胎組與侵蝕性葡萄胎間均有顯著差異(P<0.05);而以後髮生噁變的葡萄胎與侵蝕性葡萄胎間及侵蝕性葡萄胎與絨毛膜癌間無顯著差異(P>0.05)。結論 p16錶達缺失、CyclinD1過錶達可能在滋養細胞噁性轉化、侵蝕性葡萄胎髮生中起重要作用。檢測葡萄胎首次清官標本p16及CyclinD1錶達,可能在判斷葡萄胎預後方麵具有重要意義。
목적탐토p16、CyclinD1기인재자양세포종류발생방면적의의。방법용면역조화SP법,대49례자양세포질병조직(포도태수차청궁표본32례,침식성포도태급융암자궁표본분별위12례화5례)화4례조잉정상융모조직중p16화CyclinD1진행검측。결과재조잉융모조직중,pi6화CyclinD1표체분별위전부양성화전부음성。재불악변포도태、이후발생악변적포도태화자양세포종류중p16표체정하강추세,CyclinD1표체정상승추세。p16화CyclinD1표체양성솔재불악변포도태여이후발생악변적포도태조간、재불악변포도태조여침식성포도태간균유현저차이(P<0.05);이이후발생악변적포도태여침식성포도태간급침식성포도태여융모막암간무현저차이(P>0.05)。결론 p16표체결실、CyclinD1과표체가능재자양세포악성전화、침식성포도태발생중기중요작용。검측포도태수차청관표본p16급CyclinD1표체,가능재판단포도태예후방면구유중요의의。
Objective To detect the significance of p16 and CyclinD1 in the tumorigenesis of trophoblastic tumors.Methods Immunohistochemical SP method was used to detect the p16 and CyclinD1 gene expression in the tissues of 49 cases of trophoblastic diseases ( the primary curretage tissues of moles 32 cases, invasive moles 12 cases and choriocarcinoma 5 cases ) and 4 cases of normal chorion of early gestation. Results The expression of p16 and CyclinD1 was respectively all positive and all negative in the chorion of early gestation. When the moles that didn′t transform to malignancy, the moles that did transform and the trophoblasfic tumors were detected,a descending tendency of p16 expression was found ,while the expression of CyclinD1 showed an ascending tendency. The positive rate of p16 expression was significantly different between the following groups: the moles transforming malignantly later and the moles that didnt transform,the moles that didnt transform and the invasive moles( P <0.05 ) ,so was the positive rate of CyclinD1 expression. It was also observed that there wasnt significant difference between the moles that transformed later and the invasive moles, the invasive moles and the choriocarcinomas when either of the genes was detected (P>0.05). Conclusions The absent expression of the cell cycle regulating gene pi6 and the excessive expression of cyclinD1 may play a key role in the malignant transformation of trophocyte and in the development of invasive moles. Detection of p16 and Cyclin D1 expression in the primary curretage tissues of moles may be of great value in assessing the prognosis of moles.