CAJ | 학술논문

在甲肝减毒疫苗生产工艺中不同pH对疫苗得率有一定的影响，为提高疫苗的产量，在传统的氯仿抽提条件下，使用不同pH值(pH1.0～12.0)的磷酸盐缓冲体系进行氯仿抽提，检测抽提后甲肝病毒(Hepatitis A Virus，HAV)抗原滴度和感染性滴度，结果表明：经氯仿抽提后，HAV抗原滴度和感染性滴度均在pH8.0～9.0时最高，相应得率也比常规处理的得率有显著的提高.说明改进pH抽提条件对甲型肝炎减毒活疫苗后处理工艺效率的提高，成品疫苗产量的增加具有实际应用价值.
재갑간감독역묘생산공예중불동pH대역묘득솔유일정적영향，위제고역묘적산량，재전통적록방추제조건하，사용불동pH치(pH1.0～12.0)적린산염완충체계진행록방추제，검측추제후갑간병독(Hepatitis A Virus，HAV)항원적도화감염성적도，결과표명：경록방추제후，HAV항원적도화감염성적도균재pH8.0～9.0시최고，상응득솔야비상규처리적득솔유현저적제고.설명개진pH추제조건대갑형간염감독활역묘후처리공예효솔적제고，성품역묘산량적증가구유실제응용개치.
The pH plays an important role in processing technology of live attenuated hepatitis A vaccine. In order to increase te productivity of the vaccine, authors selected different pH(1.0～12.0) to study the different gaining -rate of product. The results showed that pH(8.0～9.0) were the best pH range, in this range the gaining- rate of processing thchnology was much higher than that of traditional processing.