CAJ | 학술논문

目的探索喉癌生物治疗新方法.方法建立人喉癌裸鼠模型.瘤内注射新城疫病毒.对治疗后的组织进行病理学检查、病毒分离实验,血清ELISA 实验.结果在实验组与对照组之间,鼠重(t′=2.397)、瘤重(t′=2.852)经t′检验P<0.05,瘤体积(t′=6.058) 经t′检验P<0.01,均有显著差异,抑瘤率为84.856% .病毒分离实验只有肿瘤组织中分离到病毒.ELISA实验实验组阳性.光镜和电镜下可见肿瘤细胞的坏死与凋亡及炎性细胞浸润.结论新城疫病毒治疗人喉癌裸鼠模型有效,不侵犯正常组织.
목적탐색후암생물치료신방법.방법건립인후암라서모형.류내주사신성역병독.대치료후적조직진행병이학검사、병독분리실험,혈청ELISA 실험.결과재실험조여대조조지간,서중(t′=2.397)、류중(t′=2.852)경t′검험P<0.05,류체적(t′=6.058) 경t′검험P<0.01,균유현저차이,억류솔위84.856% .병독분리실험지유종류조직중분리도병독.ELISA실험실험조양성.광경화전경하가견종류세포적배사여조망급염성세포침윤.결론신성역병독치료인후암라서모형유효,불침범정상조직.
Objective To explore the biological therapy of human laryngocarcinoma. Methods Nude mice model bearing laryngocarcinoma were established using human laryngeal squamous carcinoma cell line (Hep-II). A large of newcastle disease virus(NDV) were injected into the tumor. The microstructural and ultra-micro-structural changes of carcinoma after administration of NDV were oberserved under light and electron microscopes. NDV was isolated in all tissue. The serums were examined by the method of ELISA.Results The difference was statistically significant, the average weight of the mice: t′=2.397 P<0.05 , the average weight of the tumor tissue: t′=2.852 P<0.05 , the average volume of the tumor tissue: t′=6.058 P<0.01 . The NDV was isolated in only the tumor tissue of the experimental group .The experimental group was positive in ELISA. The necrosis and apoptosis of the tumor cell and inflammatory cellular infiltration were found under light and electron microscope. Conclusion These studies show that NDV is useful for laryngocarcinoma, that NDV is not encroach on normal tissue.