CAJ | 학술논문

目的：采用近交系小鼠，建立自体骨髓移植后免疫缺损模型，并尝试应用PCD-hIL-2注射和中药促进骨髓移植后免疫功能重建。方法：用BALB/C小鼠亚致死剂量照射后行同系骨髓移植,并用裸基因PCD-hIL-2注射与中药加味当归补血汤治疗免疫缺损。实验分组：A，中药+ PCD-hIL-2；B，PCD-hIL-2； C，中药+hIL-2；D，中药；E ，hIL-2；F，单纯BMT；G，正常对照；H，放射对照。结果：骨髓移植后10 d，发现A、B、C、D组与E、F组相比，下述参数差异有显著性（P＜0.01）：（1）脾脏、胸腺细胞总数明显增加；（2）体内NK细胞杀伤活性明显升高；（3）脾细胞、胸腺细胞、骨髓细胞对丝裂原的反应性增高；（4）混合淋巴细胞反应加强；（5）体内脾细胞产生IL-2水平及对外源性IL-2的反应性升高；（6）骨髓CFU-GM数增加。结论：PCD-hIL-2基因转移与中药均可明显促进骨髓移植后免疫缺损的恢复。
목적：채용근교계소서，건립자체골수이식후면역결손모형，병상시응용PCD-hIL-2주사화중약촉진골수이식후면역공능중건。방법：용BALB/C소서아치사제량조사후행동계골수이식,병용라기인PCD-hIL-2주사여중약가미당귀보혈탕치료면역결손。실험분조：A，중약+ PCD-hIL-2；B，PCD-hIL-2； C，중약+hIL-2；D，중약；E ，hIL-2；F，단순BMT；G，정상대조；H，방사대조。결과：골수이식후10 d，발현A、B、C、D조여E、F조상비，하술삼수차이유현저성（P＜0.01）：（1）비장、흉선세포총수명현증가；（2）체내NK세포살상활성명현승고；（3）비세포、흉선세포、골수세포대사렬원적반응성증고；（4）혼합림파세포반응가강；（5）체내비세포산생IL-2수평급대외원성IL-2적반응성승고；（6）골수CFU-GM수증가。결론：PCD-hIL-2기인전이여중약균가명현촉진골수이식후면역결손적회복。
Objective: In order to study how to accelerate the reconstitution immunofunction in BMT mice, first of all, we established a immunodeficiency model of BMT in BALB/C mice. Then BMT mice were injected with PCD-hIL-2 directly into skeletal muscle, and treated with traditional Chinese medicine. Methods: The experiment groups are designed as（A）Chinese medicine + PCD-hIL-2;（B）PCD-hIL-2;（C）Chinese medicine +hIL-2;（D）Chinese medicine;（E）hIL-2;（F）BMT;（G）normal control;（H）radiation control. Results: We compared groups A B C D to E or F groups, found（1）The splenocytes/thymocytes count increase obviously.（2）Killing activity of NK cell rises obviously in vivo.（3）The response of splenocytes、thymocytes、BM cells to mitogen goes up.（4）The reactivity of splenocytes to foreign IL-2 goes up. （5）CFU-GM count is increased. Conclusion: The expression of hIL-2 is very low by nude DNA injection ,but it is enough to have biological function and therapeutic effect .If only Chinese medicine was applied, the immunological condition was obviously recovered.