CAJ | 학술논문

肿瘤坏死因子（TNF）是一种多功能性的细胞因子，与许多重大疾病有关。为了抑制TNF的生物学活性，将TNF受体Ⅱ胞外区在大肠杆菌中进行了可溶性表达，并通过金属鳌合层析柱纯化重组蛋白。结果表明，纯化的重组蛋白能够识别并结合TNF，同时能中和TNF的生物学活性，抑制其对细胞的杀伤作用，具有潜在的临床应用前景。
종류배사인자（TNF）시일충다공능성적세포인자，여허다중대질병유관。위료억제TNF적생물학활성，장TNF수체Ⅱ포외구재대장간균중진행료가용성표체，병통과금속오합층석주순화중조단백。결과표명，순화적중조단백능구식별병결합TNF，동시능중화TNF적생물학활성，억제기대세포적살상작용，구유잠재적림상응용전경。
Tumor necrosis factor（TNF） plays an important role in vivo and isrelated to many diseases.In order to inhibit the activity of TNF，sTNFR Ⅱ was expressed in E.coli as a soluble protein and was purified by metal chelating chromatography.The purified sTNFR Ⅱ protein can bind to TNF and has the ability to neutralize the cytotoxicity of TNF.The results showed potential prospects for clinical application.