同济医科大学学报
同濟醫科大學學報
동제의과대학학보
ACTA UNIVERSITATIS MEDICINAG TONGJI
2001年
1期
46-49
,共4页
紫杉醇%脂质体%细胞毒作用%抗瘤作用
紫杉醇%脂質體%細胞毒作用%抗瘤作用
자삼순%지질체%세포독작용%항류작용
为研究紫杉醇脂质体的体外细胞毒作用及体内抗瘤作用。进行噻唑蓝（MTT）体外试验法和腹腔给药体内抗瘤试验。发现：紫杉醇脂质体对体外培养的人卵巢癌细胞COC1在浓度为22.5 μg/ml、45 μg/ml和90 μg/ml时，其抑制率分别为78.48%、84.90%和93.53%。体内腹腔给予紫杉醇脂质体5 mg/(kg*d), 10 mg/(kg*d) 8 d,对小鼠艾氏癌实体型的抑瘤率分别为32.47%和54.63%,二组有显著性差异(P<0.01);小鼠体重变化率分别为22.11%和21.16%,二组间无显著性差异(P>0.05)。结果表明:紫杉醇脂质体在体外显示细胞毒作用;体内试验显示降低药物毒性的同时保留了抗肿瘤活性。
為研究紫杉醇脂質體的體外細胞毒作用及體內抗瘤作用。進行噻唑藍（MTT）體外試驗法和腹腔給藥體內抗瘤試驗。髮現：紫杉醇脂質體對體外培養的人卵巢癌細胞COC1在濃度為22.5 μg/ml、45 μg/ml和90 μg/ml時，其抑製率分彆為78.48%、84.90%和93.53%。體內腹腔給予紫杉醇脂質體5 mg/(kg*d), 10 mg/(kg*d) 8 d,對小鼠艾氏癌實體型的抑瘤率分彆為32.47%和54.63%,二組有顯著性差異(P<0.01);小鼠體重變化率分彆為22.11%和21.16%,二組間無顯著性差異(P>0.05)。結果錶明:紫杉醇脂質體在體外顯示細胞毒作用;體內試驗顯示降低藥物毒性的同時保留瞭抗腫瘤活性。
위연구자삼순지질체적체외세포독작용급체내항류작용。진행새서람（MTT）체외시험법화복강급약체내항류시험。발현：자삼순지질체대체외배양적인란소암세포COC1재농도위22.5 μg/ml、45 μg/ml화90 μg/ml시，기억제솔분별위78.48%、84.90%화93.53%。체내복강급여자삼순지질체5 mg/(kg*d), 10 mg/(kg*d) 8 d,대소서애씨암실체형적억류솔분별위32.47%화54.63%,이조유현저성차이(P<0.01);소서체중변화솔분별위22.11%화21.16%,이조간무현저성차이(P>0.05)。결과표명:자삼순지질체재체외현시세포독작용;체내시험현시강저약물독성적동시보류료항종류활성。
The cytotoxic effect and antitumor effect of paclitaxel liposome were studied by using MTT assay method in vitro and in vivo antitumor test. The inhibitory rate of paclitaxel liposome on human ovarian cancer cell COC1 was 78.48 %, 84.90 %, 93.53 % respectively at the concentrations of 22.5 μg/ml, 45 μg/ml, 90 μg/ml respectively. Under the doses of 5 mg/(kg*d) and 10 mg/(kg*d) for 8 days, the inhibitory rate of paclitaxel liposome against xenograft of solid Ehrlich tumor in mice was 32.47 % and 54.63 % respectively (P<0.01). The weight change rate of the mice was 22.11 % and 21.16 % respectively. It was suggested that paclitaxel liposome possessed in vitro and in vivo antitumor activities. The toxicity of liposomal paclitaxel was lower than that of free paclitaxel.
