CAJ | 학술논문

用平衡透析法详细研究了pH=6.3条件下La(Ⅲ)与HSA或BSA的结合平衡.Scatchard图分析表明，La(Ⅲ)在HSA中有2个强结合部位和8个弱结合部位；在BSA中有2个强结合部位和6个弱结合部位.从La(Ⅲ)与Cu(Ⅱ)，Zn(Ⅱ)和Cd(Ⅱ)等的竞争结合HSA或BSA的结果推测：La(Ⅲ)在HSA或BSA中的一个强结合部位的配位原子可能全部是氧原子.通过非线性最小二乘法拟合Bjerrum方程，首次报道了La(Ⅲ)-HSA和La(Ⅲ)-BSA体系的逐级稳定常数值，其K1的数量级为104.Hill系数及自由能偶合分析表明La(Ⅲ)与HSA或BSA的结合均产生一定的负协同效应.
용평형투석법상세연구료pH=6.3조건하La(Ⅲ)여HSA혹BSA적결합평형.Scatchard도분석표명，La(Ⅲ)재HSA중유2개강결합부위화8개약결합부위；재BSA중유2개강결합부위화6개약결합부위.종La(Ⅲ)여Cu(Ⅱ)，Zn(Ⅱ)화Cd(Ⅱ)등적경쟁결합HSA혹BSA적결과추측：La(Ⅲ)재HSA혹BSA중적일개강결합부위적배위원자가능전부시양원자.통과비선성최소이승법의합Bjerrum방정，수차보도료La(Ⅲ)-HSA화La(Ⅲ)-BSA체계적축급은정상수치，기K1적수량급위104.Hill계수급자유능우합분석표명La(Ⅲ)여HSA혹BSA적결합균산생일정적부협동효응.
The binding of La(Ⅲ) to human serum albumin (HSA) or bovine serum albumin(BSA) has been studied by equilibrium dialysis at pH 6.3.The results from Scatchard plots indicate that there are two strong sites and eight weak sites in HSA and two strong sites and six weak sites in BSA,respectively.The results of La(Ⅲ)competing with Cu(Ⅱ),Zn(Ⅱ),Cd(Ⅱ) for binding to HSA or BSA suggest that one of the strong binding sites is most probably coordinated with atoms which are almost all oxygen.The successive stability constants which are reported for the first time are obtained by non-linear least-square method fitting Bjerrum formula.For both La (Ⅲ)-HSA and La (Ⅲ)-BSA systems,the order of magnitude of K1 was found to be 104.The analyses of Hill coefficient and free energy coupling show that some negative cooperative effect was found in both La (Ⅲ)-HSA and La (Ⅲ)-BSA systems.