CAJ | 학술논문

本研究建立了反转录聚合酶链反应（RT-PCR）技术检测猪瘟病毒（Hog CholeraVirus,HCV）方法。合成的二对引物HCV-1/HCV-2和HCV-A1/HCV-A2成功地扩增兔化弱毒株、法国株和石门株的核酸，并对采自博白县3个、贵港市1个和武宣县1个猪场共13份病料进行检测。结果从博白县和贵港市的11份材料中检测到HCV的核酸。
본연구건립료반전록취합매련반응（RT-PCR）기술검측저온병독（Hog CholeraVirus,HCV）방법。합성적이대인물HCV-1/HCV-2화HCV-A1/HCV-A2성공지확증토화약독주、법국주화석문주적핵산，병대채자박백현3개、귀항시1개화무선현1개저장공13빈병료진행검측。결과종박백현화귀항시적11빈재료중검측도HCV적핵산。
In this study reverse transcription coupled with the polymerasechain reaction(RT-PCR) has been established to detect hog cholera virus (HCV). Two pairs of primers,HCV-1/HCV-2 and HCV-A1/HCV-A2,has been synthesized and successfully to amplify nucleic acids of HCLV strain, France strain and Shimen strain of HCV. In experiment proper,13 clinical samples were examined from three differen t locolities in Guangxi namely, Bobai, Guigang and Wuxuan constituting three, one and one pig farms respectively. As a result, 11 of 13 samples from Bobai and Guigang by both primers indicating that the sick pigs in which the sample was derived were infected by HCV.