CAJ | 학술논문

目的　研究人类骨肉瘤细胞血管形成因子的作用及其生物活性。方法　通过对人类骨肉瘤组织及培养中的骨肉瘤细胞进行盐析，以及对培养中收集的大量条件培养基进行超滤、层析、透析等截留分子量8?000～10?000dal t的蛋白质纯化，获取盐析分段及19个层析分段。以人类脐静脉内皮细胞增殖反应为实验对象，用作检验该盐析和层析分段的生物活性。结果　40%的盐析分段及层析中的20ml～30ml分段具有显著的促进内皮细胞增殖的生物学效应。结论　据此表明人类骨肉瘤细胞能够合成并分泌血管形成因子，其分子量约在8?000～10?000dalt之间。
목적　연구인류골육류세포혈관형성인자적작용급기생물활성。방법　통과대인류골육류조직급배양중적골육류세포진행염석，이 급대배양중수집적대량조건배양기진행초려、층석、투석등절류분자량8?000～10?000dal t적단백질순화，획취염석분단급19개층석분단。이인류제정맥내피세포증식반응위실험대상，용작검험해염석화층석분단적생물활성。결과　40%적염석분단급층석중적20ml～30ml분단구유현저적촉진내 피세포증식적생물학효응。결론　거차표명인류골육류세포능구합성병분비혈관형성인자，기 분자량약재8?000～10?000dalt지간。
Objective To partially purify the angiogenesis factor of human osteosarcoma (HuOs) and study its biological features.Methods The active peptide with a molecular weight of 8*!000-10*!000 Da in the conditioned medium obtained from the cultivation of HuOs cells (osteoblastic osteosarcoma) was partially purified by ultrafiltration, chromatography and dialysis. The angiogenic effects of the fractions were assessed by proliferation assay of human umbilical vein and pig thoracic aorta endothelial cells. Results The chromatography fractions 4-6 could significantly promote the proliferation of the endothelial cells.Conclusion The HuOs cells could synthesize and secrete angiogenesis factor with a molecular weight of 8000-10000 Da.