CAJ | 학술논문

用定位候选克隆法克隆了一个与鼻咽癌相关的基因,命名为NAG73基因. 结构分析显示NAG73的基因序列无内含子, 无典型的启动子序列, poly A尾.与人类生长激素促分泌因子基因的第4个外显子和3′端非翻译区同源.说明NAG73可能是人类生长激素促分泌因子基因的假基因. 同时,实验证明NAG73在一些细胞和组织中活跃表达.在正常鼻咽上皮细胞,鼻咽癌上皮细胞和鼻咽癌活检组织,NAG73有表达差异.序列分析显示NAG73有编码翻译产物的可能.NAG73可能可编码产物.该产物在鼻咽癌的发病发展中发挥作用.
용정위후선극륭법극륭료일개여비인암상관적기인,명명위NAG73기인. 결구분석현시NAG73적기인서렬무내함자, 무전형적계동자서렬, poly A미.여인류생장격소촉분비인자기인적제4개외현자화3′단비번역구동원.설명NAG73가능시인류생장격소촉분비인자기인적가기인. 동시,실험증명NAG73재일사세포화조직중활약표체.재정상비인상피세포,비인암상피세포화비인암활검조직,NAG73유표체차이.서렬분석현시NAG73유편마번역산물적가능.NAG73가능가편마산물.해산물재비인암적발병발전중발휘작용.
A nasopharyngeal carcinoma(NPC) related gene, named NAG73 which mapped to 3p25～26 was identified by positional candidate cloning. Structural analysis reveals that NAG73 has an intronless genomic sequence, which is homologous with the fourth exon and 3′UTR of Homo sapiens Growth Hormone Secretagogue Precursor Gene (GHRELIN). NAG73 has no 5′ promoter sequence, and has putative polyA. The results suggest that NAG73 might be a processed pseudogene of GHRELIN. It has been showed that NAG73 is actively transcribed in some cells and tissues examined. There is expression difference between normal nasopharynx epithelia and nasopharyngeal carcinoma epithelia. And there is potential for a NAG73 encoded translation product. So, it is possible that NAG73 is translated and the product might act on the tumorigenesis of nasopharyngeal carcinoma.