CAJ | 학술논문

普通野生稻是栽培稻的祖先种,其遗传多样性远远大于栽培稻,蕴涵着栽培品种中难以找到的重要性状.以江西东乡普通野生稻为供体、以桂朝2号为遗传背景的野生稻基因渗入系(BC4F5、BC4F6)为材料,利用30%的PEG人工模拟干旱环境,对渗入系二叶一心苗期进行抗旱鉴定,共定位了12个与抗旱有关的QTL,其中在第2、6和12染色体上发现了4个QTL的加性效应值为正,来自东乡野生稻的等位基因能使渗入系的抗旱性增强,特别是位于第12染色体RM17附近的qSDT12-2在多次重复中均被检测到,在PEG处理后1-8 d能稳定表达.通过对抗旱性QTL的动态分析,发现不同QTL表达时间不同.
보통야생도시재배도적조선충,기유전다양성원원대우재배도,온함착재배품충중난이조도적중요성상.이강서동향보통야생도위공체、이계조2호위유전배경적야생도기인삼입계(BC4F5、BC4F6)위재료,이용30%적PEG인공모의간한배경,대삼입계이협일심묘기진행항한감정,공정위료12개여항한유관적QTL,기중재제2、6화12염색체상발현료4개QTL적가성효응치위정,래자동향야생도적등위기인능사삼입계적항한성증강,특별시위우제12염색체RM17부근적qSDT12-2재다차중복중균피검측도,재PEG처리후1-8 d능은정표체.통과대항한성QTL적동태분석,발현불동QTL표체시간불동.
Common wild rice (Oryza rufipogon Griff.) is the ancestor of cultivated rice (O.sativa L.), which has a greater genetic diversity and important traits that remain to be employed in cultivated rice. In this study, a set of introgression lines (BC4F5 and/or BC4F6) carrying various introgressed segments from common wild rice, collected from Dongxiang county, Jiangxi Province, China,in the background of an Indica (O. sativa L. ssp. indica) cultivar, Guichao 2, was used. A total of 12 drought-related quantitative trait loci (QTL) were identified by investigating drought tolerance of introgression lines under 30% PEG treatment at the young seedlings stage. Of these QTLs, the alleles of 4 QTLs on chromosome 2, 6 and 12 from Dongxiang common wild rice were responsible for increased drought tolerance of the introgression lines. In particular, a QTL qSDT12-2, near RM17 on chromosome 12, was consistently detected in different replications, and expressed stably under PEG stress throughout the study. It was also found that the QTLs located on different chromosomes might express at different stages.