当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2014年
13期
17-19
,共3页
王翠华%潘春霞%王伟%蔡永清
王翠華%潘春霞%王偉%蔡永清
왕취화%반춘하%왕위%채영청
肿瘤%多药耐药性%逆转%干扰素%MX-1/T
腫瘤%多藥耐藥性%逆轉%榦擾素%MX-1/T
종류%다약내약성%역전%간우소%MX-1/T
Cancer%Multidrug resistance%Reverse%Interferon%MX-1/T
目的：探讨不同浓度的α-干扰素(alpha-interferon,IFN-α)对人乳腺癌细胞株MX-1及耐紫杉醇的人乳腺癌耐药细胞株MX-1/Taxol(MX-1/T)的影响。方法以四甲基偶氮唑盐比色法(methyl thiazolyl tetrazolium,MTT)测定梯度浓度的干扰素(IFN-α)及紫杉醇（taxol,TAX)的细胞毒性变化。以免疫印迹试验测定IFN-α作用后肿瘤细胞P-糖蛋白（P-glycoprotein，P-gp）、多药耐药相关蛋白(multidrug resistance protein,MRP)、谷胱甘肽S-转移酶（gultathione S-transferases，GST）的变化。结果 MX-1/T对TAX的耐药倍数为21.9倍。加入不同浓度IFN-a(700 U/mL、1400 U/mL)作用于MX-1/T细胞后，其逆转耐药倍数分别为10.2和12.7与MX-1/T和MX-1/T+紫杉醇(400 ng/mL)组相比，加入不同浓度的IFN-α作用后的MX-1/T细胞中MRP、P-gp、GST的阳性带强度减弱，条带变细，但700 U/mL和1400 U/mL组无明显差别。结论 IFN-α对MX-1/T细胞的多药耐药性有明显的逆转作用，且在一定浓度范围内有剂量依赖性。
目的：探討不同濃度的α-榦擾素(alpha-interferon,IFN-α)對人乳腺癌細胞株MX-1及耐紫杉醇的人乳腺癌耐藥細胞株MX-1/Taxol(MX-1/T)的影響。方法以四甲基偶氮唑鹽比色法(methyl thiazolyl tetrazolium,MTT)測定梯度濃度的榦擾素(IFN-α)及紫杉醇（taxol,TAX)的細胞毒性變化。以免疫印跡試驗測定IFN-α作用後腫瘤細胞P-糖蛋白（P-glycoprotein，P-gp）、多藥耐藥相關蛋白(multidrug resistance protein,MRP)、穀胱甘肽S-轉移酶（gultathione S-transferases，GST）的變化。結果 MX-1/T對TAX的耐藥倍數為21.9倍。加入不同濃度IFN-a(700 U/mL、1400 U/mL)作用于MX-1/T細胞後，其逆轉耐藥倍數分彆為10.2和12.7與MX-1/T和MX-1/T+紫杉醇(400 ng/mL)組相比，加入不同濃度的IFN-α作用後的MX-1/T細胞中MRP、P-gp、GST的暘性帶彊度減弱，條帶變細，但700 U/mL和1400 U/mL組無明顯差彆。結論 IFN-α對MX-1/T細胞的多藥耐藥性有明顯的逆轉作用，且在一定濃度範圍內有劑量依賴性。
목적：탐토불동농도적α-간우소(alpha-interferon,IFN-α)대인유선암세포주MX-1급내자삼순적인유선암내약세포주MX-1/Taxol(MX-1/T)적영향。방법이사갑기우담서염비색법(methyl thiazolyl tetrazolium,MTT)측정제도농도적간우소(IFN-α)급자삼순（taxol,TAX)적세포독성변화。이면역인적시험측정IFN-α작용후종류세포P-당단백（P-glycoprotein，P-gp）、다약내약상관단백(multidrug resistance protein,MRP)、곡광감태S-전이매（gultathione S-transferases，GST）적변화。결과 MX-1/T대TAX적내약배수위21.9배。가입불동농도IFN-a(700 U/mL、1400 U/mL)작용우MX-1/T세포후，기역전내약배수분별위10.2화12.7여MX-1/T화MX-1/T+자삼순(400 ng/mL)조상비，가입불동농도적IFN-α작용후적MX-1/T세포중MRP、P-gp、GST적양성대강도감약，조대변세，단700 U/mL화1400 U/mL조무명현차별。결론 IFN-α대MX-1/T세포적다약내약성유명현적역전작용，차재일정농도범위내유제량의뢰성。
Objective To explore the effects of various concentrations alpha-interferon(IFN-α)on human breast cancer cell lines MX-1 and multidrug resistance cell lines MX-1/Taxol(MX-1/T). Methods The toxicity variation of IFN-αand Taxol (TAX) was studied by methyl thiazolyl tetrazolium (MTT) assay. And the influence of IFN-α on variation of P-glycoprotein (P-gp)、Multidrug resistance protein (MRP)、Gultathione S-transferases (GST) cancer cell were measured by immunoblotting. Results Drug-resistant multiples of MX-1/T to TAX is 21.9. Different concentration (IFN-α 700 U/mL, 1400 U/mL) effect on MX-1/T cells, the reversal drug-resistant multiples are 10.2 and 1.27 respectively. Compared with MX-1/T and MX-1/T+TAX (400 ng/mL) group, different concentrations IFN-αdecrease masculine gender of MRP, P-gp, GST in MX-1/T cells, strip attenuate, but 700 U/mL and 1400 U/mL group has no obvious difference. Conclusion IFN-αhassignificant reversal effect of multidrug resistance MX-1/T cell lines, and it has dose dependent in certain concentration range.
