东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2009年
10期
43-48
,共6页
任焕%王宏燕%孟杰%韩晓盈
任煥%王宏燕%孟傑%韓曉盈
임환%왕굉연%맹걸%한효영
耐冷菌%生物表面活性剂%筛选
耐冷菌%生物錶麵活性劑%篩選
내랭균%생물표면활성제%사선
psychrotrophic bacteria%biosurfactant%screening
从常年堆积的秸秆堆下面的土样和牛场的堆肥中,筛选出13株能产生生物表面活性剂的细菌.其中有6株菌株为耐冷菌,其发酵液在低温环境下(0~10℃)仍具有较低的表面张力,且均属于假单胞菌属(Pseudomonas),其余属于土壤杆菌属(Agrobacterium).对一株耐冷菌G3-6进行最佳发酵培养基组成和发酵条件进行了研究.经鉴定,其代谢产物是糖脂类.同时也对生物表面活性剂在作物生长中的作用进行了初步的研究.
從常年堆積的秸稈堆下麵的土樣和牛場的堆肥中,篩選齣13株能產生生物錶麵活性劑的細菌.其中有6株菌株為耐冷菌,其髮酵液在低溫環境下(0~10℃)仍具有較低的錶麵張力,且均屬于假單胞菌屬(Pseudomonas),其餘屬于土壤桿菌屬(Agrobacterium).對一株耐冷菌G3-6進行最佳髮酵培養基組成和髮酵條件進行瞭研究.經鑒定,其代謝產物是糖脂類.同時也對生物錶麵活性劑在作物生長中的作用進行瞭初步的研究.
종상년퇴적적갈간퇴하면적토양화우장적퇴비중,사선출13주능산생생물표면활성제적세균.기중유6주균주위내랭균,기발효액재저온배경하(0~10℃)잉구유교저적표면장력,차균속우가단포균속(Pseudomonas),기여속우토양간균속(Agrobacterium).대일주내랭균G3-6진행최가발효배양기조성화발효조건진행료연구.경감정,기대사산물시당지류.동시야대생물표면활성제재작물생장중적작용진행료초보적연구.
Thirteen biosurfactant-producing micro-organisms were isolated from the soil of cattle farm and the soil under the heap of straw for all the years. Six strains were psychrotrophic bacteria in the thirteen, the fermentation liquid still had lower surface tension in low temperature (0-10 ℃), and they all belonged to the Pseudomonas, the rest seven strains were Agrobacterium. In addition, the optimization for the best fermented medium conditions of strain G3-6 was studied. The metabolites of G3-6 was proved as glycolipid. And also the article studied the role of biosurfactant in the plant growth.