CAJ | 학술논문

应用分子系统发育学的方法, 以浙江沿海一海地瓜的18S rDNA和COI片段序列为分子标记,自行设计引物,进行了克隆, 结合来自GenBank 中10种海参的18S rDNA和COI 同源序列, 用邻接法和最大简约法构建系统进化树,并结合形态学特征对它们的分类地位进行了探讨.初步分析结果,基于此海地瓜的各形态学的研究,可以认为它属于海参纲芋参目;鉴于18S rDNA基因的分析,此海地瓜与拟刺参遗传距离为0.017亲缘关系非常近,可以认为它属于海参纲;从COI基因的角度分析,它虽然与怀玉参在系统树上聚成一枝,但支持率很低;仅为49%,遗传距离比较大,为0.223,不足以作为分析的依据.
응용분자계통발육학적방법, 이절강연해일해지과적18S rDNA화COI편단서렬위분자표기,자행설계인물,진행료극륭, 결합래자GenBank 중10충해삼적18S rDNA화COI 동원서렬, 용린접법화최대간약법구건계통진화수,병결합형태학특정대타문적분류지위진행료탐토.초보분석결과,기우차해지과적각형태학적연구,가이인위타속우해삼강우삼목;감우18S rDNA기인적분석,차해지과여의자삼유전거리위0.017친연관계비상근,가이인위타속우해삼강;종COI기인적각도분석,타수연여부옥삼재계통수상취성일지,단지지솔흔저;부위49%,유전거리비교대,위0.223,불족이작위분석적의거.
The phylogenetic relation of sea cucumber Acaudina molpadioidea from the coast in Zhejiang province were studied by analysis of 1312 bp from 18S rDNA and 692 bp from COImt DNA with homologous sequences of ten species within Holothuroidea downloaded from GenBank as well as the morphological characteristics. Molecular phylogenetic trees were constructed by Neighbor-Joining(NJ)and Maximum Parsimony(MP). The sea cucumber was found to be to Molpadiidae Holothuroidea, inferred from its Bio-Morphotyping. The 18S rDNA gene analysis reveals that the genetic distance between this sea cucumber and Pseudostichopus villosus is 0.017, that Acaudina molpadioidea belongs to Holothuroidea and is close to the Holothuroidea, Aspidochirota. Based on the COImt DNA gene analysis, there is close relationship between Acaudina molpadioidea and Pentamera lissoplaca, with long genetic distance of 0.223, not being the basis for the phylogenetic position.