安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2010年
7期
3545-3547
,共3页
牛%附红细胞体%体外%药物筛选
牛%附紅細胞體%體外%藥物篩選
우%부홍세포체%체외%약물사선
Cattle%Eperythrozoon%in vitro%Drug screening
[目的]筛选对温氏附红细胞体体外杀灭具有最佳效果的药物. [方法]以RPMI-1640为基础培养液,加入30%犊牛血清,选用盐酸土霉素、恩诺沙星、磺胺对甲氧嘧啶、硫酸庆大霉素、磷酸伯氨喹啉、盐酸咪唑苯脲、贝尼尔7种药物,在37 ℃、5%CO_2的环境下,进行温氏附红细胞体体外药物杀灭试验.[结果]磷酸伯氨喹、盐酸咪唑苯脲对牛附红细胞体的体外杀灭效果最佳,当培养液药物浓度为2 000 μg/ml,药物与附红细胞体作用6 h时,红细胞染虫率显著下降到30%以下.盐酸土霉素、恩诺沙星、贝尼尔和磺胺对甲氧嘧啶对附红细胞体均有一定作用.硫酸庆大霉素具有较强抗菌作用,但对牛附红细胞体病无效.[结论]该研究为临床选择抗附红细胞体病的合适药物提供理论依据.
[目的]篩選對溫氏附紅細胞體體外殺滅具有最佳效果的藥物. [方法]以RPMI-1640為基礎培養液,加入30%犢牛血清,選用鹽痠土黴素、恩諾沙星、磺胺對甲氧嘧啶、硫痠慶大黴素、燐痠伯氨喹啉、鹽痠咪唑苯脲、貝尼爾7種藥物,在37 ℃、5%CO_2的環境下,進行溫氏附紅細胞體體外藥物殺滅試驗.[結果]燐痠伯氨喹、鹽痠咪唑苯脲對牛附紅細胞體的體外殺滅效果最佳,噹培養液藥物濃度為2 000 μg/ml,藥物與附紅細胞體作用6 h時,紅細胞染蟲率顯著下降到30%以下.鹽痠土黴素、恩諾沙星、貝尼爾和磺胺對甲氧嘧啶對附紅細胞體均有一定作用.硫痠慶大黴素具有較彊抗菌作用,但對牛附紅細胞體病無效.[結論]該研究為臨床選擇抗附紅細胞體病的閤適藥物提供理論依據.
[목적]사선대온씨부홍세포체체외살멸구유최가효과적약물. [방법]이RPMI-1640위기출배양액,가입30%독우혈청,선용염산토매소、은낙사성、광알대갑양밀정、류산경대매소、린산백안규람、염산미서분뇨、패니이7충약물,재37 ℃、5%CO_2적배경하,진행온씨부홍세포체체외약물살멸시험.[결과]린산백안규、염산미서분뇨대우부홍세포체적체외살멸효과최가,당배양액약물농도위2 000 μg/ml,약물여부홍세포체작용6 h시,홍세포염충솔현저하강도30%이하.염산토매소、은낙사성、패니이화광알대갑양밀정대부홍세포체균유일정작용.류산경대매소구유교강항균작용,단대우부홍세포체병무효.[결론]해연구위림상선택항부홍세포체병적합괄약물제공이론의거.
[Objective] The study aimed to screen the medicines that had the optimum killing effects on Eperythrozoon wenyonii in vitro. [Method] With RPMI-1640 as the basic medium, adding 30% calf serum, the 7 medicines including oxytetracycline hydrochloride, enrofloxacin, sulfonamides methoxy-pyrimidine, gentamicin sulfate, primaquine phosphate morpholine, zmizol and bernier were selected to make for the medicine killing test on E. wenyonii in vitro under 5% CO_2 environment at 37 ℃.[Result] The primaquine phosphate and zmizol had best killing effects on E. wenyonii in vitro. When their concn. in the cultured liquid was 2 000 μg/ml and these two medicines acted with E. wenyonii for 6 h, the infestation ratio of erythrocyte was obviously decreased by below 30%. The oxytetracycline hydrochloride, enrofloxacin, bernier and sulfonamides had some action on the eperythrozoon. The gentamicin sulfate had stronger antibacterial effect, but it showed no killing effect on E. wenyonii. [Conclusion] This study provided the theoretical basis on selecting suitable medicine resisting eperythrozoon in the clinic.