CAJ | 학술논문

在已报道的番木瓜果胶裂解酶(PL)和β-半乳糖苷酶(β-Gal)基因序列的基础上,设计特异引物,分别扩增保守区序列,将其分别反向插入植物表达载体pCAMBIA1301-35S-GUS-Nos的CaMV35S启动子和Nos终止子之间,构建反义表达载体pCP和pCG.然后用2种不同的方法将带有完整启动子和终止子的PL和β-Gal基因引入pCAMBIA2301中,获得PL和β-Gal基因的双价植物反义表达载体pCPG,并对2种方法进行比较.
재이보도적번목과과효렬해매(PL)화β-반유당감매(β-Gal)기인서렬적기출상,설계특이인물,분별확증보수구서렬,장기분별반향삽입식물표체재체pCAMBIA1301-35S-GUS-Nos적CaMV35S계동자화Nos종지자지간,구건반의표체재체pCP화pCG.연후용2충불동적방법장대유완정계동자화종지자적PL화β-Gal기인인입pCAMBIA2301중,획득PL화β-Gal기인적쌍개식물반의표체재체pCPG,병대2충방법진행비교.
Pectate lyase and β-Galactosidase (β-Gal) are important softening enzyme. The transcriptions of PL and β-Gal gene were inhibited by antisense technique may put off papaya (Carica papaya L.) fruit softening. PL and β-Gal conserved domains were cloned using specific primers designed according to those published genes. The PL and β-Gal cDNA were inserted into between the CaMV35S promoter and Nos terminator of the expression vector pCAMBIA1301-35S-GUS-Nos with reverse orientation, respectively. So, two antisense expression vectors pCP and pCG were obtained. Then, PL and β-Gal antisense genes with 35S promoter and Nos terminator were inserted into expression vector pCAMBIA2301 together to generate double genes plant expression vector pCPG by different mode.