浙江大学学报（农业与生命科学版）
浙江大學學報（農業與生命科學版）
절강대학학보（농업여생명과학판）
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2010年
2期
125-132
,共8页
大肠杆菌%耐药表型%肠杆菌科基因间重复一致(ERIC)序列%聚类分析
大腸桿菌%耐藥錶型%腸桿菌科基因間重複一緻(ERIC)序列%聚類分析
대장간균%내약표형%장간균과기인간중복일치(ERIC)서렬%취류분석
Escherichia coli%drug-resistant phenotype%enterobacterial repetitive intergenic consensus (ERIC) sequence%cluster analysis
以47株黄白痢临床分离大肠杆菌为材料,通过Kirby-Bauer法检测细菌耐药表型并用PCR扩增Ⅰ型整合酶基因,进而对不同耐药谱菌株进行以肠杆菌科基因间重复一致序列为引物的聚合酶链反应(ERIC-PCR)基因分型并用统计分析软件SPSS 16.0聚类.耐药表型检测结果显示:47株大肠杆菌均呈多重耐药,共9种耐药表型;Ⅰ型整合子阳性菌株检出率为95.7%(45/47);ERIC-PCR扩增出现稳定可重复的基因指纹图谱,耐9～13种药物菌株(85.1%,40/47)的ERIC指纹图谱分别显示出2种主要谱型,各代表1个猪场的菌株类型.聚类分析提示,相同耐药谱的来自同一猪场和不同猪场分离株的平均相似率分别为68.7%和53.5%,显著高于47株菌的平均相似率37.3%.说明菌株耐药性可能与特定的ERIC指纹图谱相关,ERIC指纹图谱分析可作为考察猪源致病性大肠杆菌多重耐药表型与基因型特征的新视角.
以47株黃白痢臨床分離大腸桿菌為材料,通過Kirby-Bauer法檢測細菌耐藥錶型併用PCR擴增Ⅰ型整閤酶基因,進而對不同耐藥譜菌株進行以腸桿菌科基因間重複一緻序列為引物的聚閤酶鏈反應(ERIC-PCR)基因分型併用統計分析軟件SPSS 16.0聚類.耐藥錶型檢測結果顯示:47株大腸桿菌均呈多重耐藥,共9種耐藥錶型;Ⅰ型整閤子暘性菌株檢齣率為95.7%(45/47);ERIC-PCR擴增齣現穩定可重複的基因指紋圖譜,耐9～13種藥物菌株(85.1%,40/47)的ERIC指紋圖譜分彆顯示齣2種主要譜型,各代錶1箇豬場的菌株類型.聚類分析提示,相同耐藥譜的來自同一豬場和不同豬場分離株的平均相似率分彆為68.7%和53.5%,顯著高于47株菌的平均相似率37.3%.說明菌株耐藥性可能與特定的ERIC指紋圖譜相關,ERIC指紋圖譜分析可作為攷察豬源緻病性大腸桿菌多重耐藥錶型與基因型特徵的新視角.
이47주황백리림상분리대장간균위재료,통과Kirby-Bauer법검측세균내약표형병용PCR확증Ⅰ형정합매기인,진이대불동내약보균주진행이장간균과기인간중복일치서렬위인물적취합매련반응(ERIC-PCR)기인분형병용통계분석연건SPSS 16.0취류.내약표형검측결과현시:47주대장간균균정다중내약,공9충내약표형;Ⅰ형정합자양성균주검출솔위95.7%(45/47);ERIC-PCR확증출현은정가중복적기인지문도보,내9～13충약물균주(85.1%,40/47)적ERIC지문도보분별현시출2충주요보형,각대표1개저장적균주류형.취류분석제시,상동내약보적래자동일저장화불동저장분리주적평균상사솔분별위68.7%화53.5%,현저고우47주균적평균상사솔37.3%.설명균주내약성가능여특정적ERIC지문도보상관,ERIC지문도보분석가작위고찰저원치병성대장간균다중내약표형여기인형특정적신시각.
In order to investigate the relationship between fingerprinting and antimicrobial resistance patterns, 47 isolates of Escherichia coli from piglets with pre-weaning diarrhea from 2 local large piggeries were collected for susceptibility testing by Kirby-Bauer method and studied the presence of integron integrase gene by polymerase chain reaction (PCR), respectively. The isolates with different phenotypes were then genotyped by enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting and further clustered on the basis of the profiles using statistical software SPSS 16.0. The results showed that the 47 isolates were all multi-drug resistant and exhibited 9 phenotypes, whereas 45 of the 47 isolates (95.7%) were integron I positive. ERIC-PCR was found to generate the reproducible fingerprinting profiles; two dominant profiles were shown in fingerprints of isolates resistant to 9-13 antimicrobial agents, each standing for isolates from the relevant pig farm. The cluster analysis demonstrated that the similarity among the isolates with the same patterns of resistance from the same piggery and different piggeries was 68.7% and 53.5%, respectively, significantly (P<0.05) higher than that of 47 isolates (37.3%). It is indicated that specific ERIC fingerprints might have relations with resistance pattern, and ERIC-PCR fingerprinting could be applied as a novel tool to the characterizations of multi-resistant phenotypes and genotypes of E. coli from piglets with diarrhea.
