CAJ | 학술논문

目的:检测P73蛋白和p73 mRNA在反应性增生性淋巴结炎(reactive hyperplastic lymphadenitis, RHL)及滤泡性淋巴瘤(follicular lymphoma, FL)中的表达.同时对p73基因的甲基化状态进行研究,初步探讨p73基因和基因修饰状态在RHL和FL发生发展中的作用. 方法:应用免疫组化法检测20例RHL及18例FL中P73蛋白的表达情况.用逆转录聚合酶链反应技术(RT-PCR)检测p73mRNA在RHL、PL中的表达情况.采用甲基化特异性PCR(MSP)方法检测RHL、PL中p73基因第1外显子区域5'-CpG岛的甲基化状态. 结果:12例FL(12/18,66.7%)和16例RHL(16/20,80%)P73蛋白表达呈阳性.P73蛋白在PL中的阳性率与RHL相比差别无统计学意义(P>0.05).RT-PCR结果显示p73基因在FL中的阳性表达率稍低于RHL,但差异无统计学意义(P>0.05).甲基化检测结果显示FL中仅有2例(2/18,11.1%)出现p73基因甲基化,而这2例标本均未表达p73 mRNA及蛋白.在RHL中,未检测到p73基因甲基化.结论:p73基因在FL和RHL中的表达无明显差别,且其甲基化在二者中并不常见.
목적:검측P73단백화p73 mRNA재반응성증생성림파결염(reactive hyperplastic lymphadenitis, RHL)급려포성림파류(follicular lymphoma, FL)중적표체.동시대p73기인적갑기화상태진행연구,초보탐토p73기인화기인수식상태재RHL화FL발생발전중적작용. 방법:응용면역조화법검측20례RHL급18례FL중P73단백적표체정황.용역전록취합매련반응기술(RT-PCR)검측p73mRNA재RHL、PL중적표체정황.채용갑기화특이성PCR(MSP)방법검측RHL、PL중p73기인제1외현자구역5'-CpG도적갑기화상태. 결과:12례FL(12/18,66.7%)화16례RHL(16/20,80%)P73단백표체정양성.P73단백재PL중적양성솔여RHL상비차별무통계학의의(P>0.05).RT-PCR결과현시p73기인재FL중적양성표체솔초저우RHL,단차이무통계학의의(P>0.05).갑기화검측결과현시FL중부유2례(2/18,11.1%)출현p73기인갑기화,이저2례표본균미표체p73 mRNA급단백.재RHL중,미검측도p73기인갑기화.결론:p73기인재FL화RHL중적표체무명현차별,차기갑기화재이자중병불상견.
OBJECTIVE: To detect the expression of P73 protein and p73 Mrna in reactive hyperplasitic lymphadenitis (RHL) and follicular lymphomas (FL) . To explore the relationship between p73 gene and its methylation status. To explore and discuss the possible roles of p73 gene in the development and progression of RHL and FL.METHODS: P73 protein expression was detected in 20 cases of RHL and 18 cases of FL by immunohistochemistry. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to observe p73 mRNA expression. We also used the methylation-specific polymerase chain reaction (MSP) to detect the methylation state of p73 gene CD, FL and RHL.RESULTS: P73 protein expression was positive in 12 of 18 FL and 16 of 20 in RHL by immunohistochemistry. The expression rates of p73 mRNA were the same in FL and RHL(P > 0.05). Methylation of p73 gene was found in 2 cases of 18 FL(11.1%), while p73 MRNA and protein expression were not found in these. No methylation of p73 gene was found in FL. CONCLUSION: There was no statistically significant differences of p73 expression between RHL and FL. Methylation of p73 was uncommon in FL and RHL.