CAJ | 학술논문

[目的]研究不同浓度的钼对甘草生长、产量和有效成分积累的影响.[方法]以一年生的甘草移栽苗为试验材料,采用盆栽蛭石的试验方法,共设置4个钼浓度水平,分别为0,0.52,5.20 和 10.40 mg/L,其中0.52 mg/L 即正常Hoagland营养液中钼的浓度.每周向盆内浇灌营养液,以达到处理的目的.采用电子天平分别测定不同处理时间下的甘草地上、根的鲜重和干重.按照<中国药典>(2010年版,一部)应用HPLC测定甘草根主要有效成分甘草酸和甘草苷的含量.[结果]结果表明,甘草地上部分、根的鲜重和干重均随钼处理浓度的增加而增加,试验处理后期差异显著.在处理105 d时,在0 mg/L处理下甘草各部位的干、鲜重最低,5.20 和 10.40 mg/L处理下最高.然而,随着钼处理浓度的增加,甘草酸和甘草苷含量呈现出先增加后降低的趋势.在整个试验过程中,钼浓度为5.20 mg/L处理下的甘草酸和甘草苷含量始终高于其他处理.[结论]5.20和 10.40 mg/L的钼浓度可以促进甘草地上和根增加,但是5.20 mg/L钼更适合根中活性成分甘草酸和甘草苷的积累.
[목적]연구불동농도적목대감초생장、산량화유효성분적루적영향.[방법]이일년생적감초이재묘위시험재료,채용분재질석적시험방법,공설치4개목농도수평,분별위0,0.52,5.20 화 10.40 mg/L,기중0.52 mg/L 즉정상Hoagland영양액중목적농도.매주향분내요관영양액,이체도처리적목적.채용전자천평분별측정불동처리시간하적감초지상、근적선중화간중.안조<중국약전>(2010년판,일부)응용HPLC측정감초근주요유효성분감초산화감초감적함량.[결과]결과표명,감초지상부분、근적선중화간중균수목처리농도적증가이증가,시험처리후기차이현저.재처리105 d시,재0 mg/L처리하감초각부위적간、선중최저,5.20 화 10.40 mg/L처리하최고.연이,수착목처리농도적증가,감초산화감초감함량정현출선증가후강저적추세.재정개시험과정중,목농도위5.20 mg/L처리하적감초산화감초감함량시종고우기타처리.[결론]5.20화 10.40 mg/L적목농도가이촉진감초지상화근증가,단시5.20 mg/L목경괄합근중활성성분감초산화감초감적적루.
[Objective] The aim was to explore the effects of different concentrations of molybdenum (Mo) on the growth, yield and the accumulations of active constituents of Glycyrrhiza uralensis.[Method] The seedlings of one-year-old G.uralensis were subjected to four concentrations of Mo (0, 0.52, 5.20 and 10.40 mg/L Mo) culturing in vermiculite and 0.52 mg/L Mo was the normal concentration in the complete Hoagland nutrition solution.The solution was irrigated to the pots every week.The fresh and dry weights of shoots and roots of the plants were measured by electronic balance.The contents of glycyrrhizic acid and liquiritin of roots were determined by HPLC.[Result] This result showed that both fresh and dry weights of the shoots and roots increased with the increasing of Mo concentrations.During the later period of this experiment, they showed significant differences among different Mo treatments.On the 105 d, both fresh and dry weights of the shoots and roots under 0 mg/L Mo reached the lowest, and the 5.20 and 10.40 mg/L Mo reached the highest.However, the contents of glycyrrhizic acid and liquiritin increased first and then decreased with increasing of Mo concentrations.During the whole experiment, the contents of glycyrrhizic acid and liquiritin under 5.20 mg/L Mo were always higher than that under other Mo treatments.[Conclusion] In conclusion, both the concentrations of 5.20 and 10.40 mg/L Mo could promote the accumulation of the shoots and roots of G.uralensis, but the 5.20 mg/L Mo was more useful for the accumulation of glycyrrhizic acid and liquiritin in the roots.