CAJ | 학술논문

目的研究CTLA4Ig基因在糖尿病大鼠体内表达及其产物对胰岛移植物存活的作用.方法利用Lipofectin载体包裹CTLA41g cDNA 质粒后转染鼠胰岛和肌肉细胞,检测移植后CTLA4Ig表达和T淋巴细胞转化率.结果胰岛移植术后7dT淋巴细胞转化试验,实验组(A组)和对照组(B组)每分钟脉冲数(cpm)分别为175.7±98.2,254.4±116.3,两组比较差异显著(P＜0.05).A组胰岛移植第7d,2只大鼠血清CTLA4Ig呈阳性(阳性率20%).A、B两组胰岛移植后血糖维持正常时间分别为(14.8±12.3)d和(3.6±5.1)d,两组比较差异显著(P＜0.05).A、B两组大鼠平均存活时间分别为(24.0±10.8)d和(10.8±4,8)d,两组比较,差异有极显著性(P＜0.01).结论脂质体包裹的CTLA41g cDNA转染肌细胞和胰岛细胞,可以在受体大鼠胰岛细胞或肌肉组织中表达,其表达产物可使胰岛移植物和受体鼠存活时间明显延长,抑制细胞免疫活性,发挥其治疗排斥反应的作用.
목적 연구CTLA4Ig기인재당뇨병대서체내표체급기산물대이도이식물존활적작용.방법 이용Lipofectin재체포과CTLA41g cDNA 질립후전염서이도화기육세포,검측이식후CTLA4Ig표체화T림파세포전화솔.결과 이도이식술후7dT림파세포전화시험,실험조(A조)화대조조(B조)매분종맥충수(cpm)분별위175.7±98.2,254.4±116.3,량조비교차이현저(P＜0.05).A조이도이식제7d,2지대서혈청CTLA4Ig정양성(양성솔20%).A、B량조이도이식후혈당유지정상시간분별위(14.8±12.3)d화(3.6±5.1)d,량조비교차이현저(P＜0.05).A、B량조대서평균존활시간분별위(24.0±10.8)d화(10.8±4,8)d,량조비교,차이유겁현저성(P＜0.01).결론 지질체포과적CTLA41g cDNA전염기세포화이도세포,가이재수체대서이도세포혹기육조직중표체,기표체산물가사이도이식물화수체서존활시간명현연장,억제세포면역활성,발휘기치료배척반응적작용.
Objectives To study the expression of CTLA4Ig gene in diabetic rat and the action of CTLA4Ig on prolonging survival time of the pancreatic islet.Methods The rat pancreatic islet cells and muscle cells were transfected with the cDNA for CTLA4Ig packaged with lipofectin vector.The expression levels of CTLA4Ig gene and T lymphocyte reaction(MLR)were determined and the action of CTLA4IgcDNA on prolonging survival time of the pancreatic islet allograft in diabetic rats observed.Results T lymphocyte transforming test from peripheral intravenous blood at 7 th day after pancreatic islet transplantationrevealed that cpm in group A and group B were 175.7±98.2,254.4±116.3,respectively,with the difference between the two groups being significant(P＜0.05).At 7th day after pancreatic islet allograft in group A,only 2 out of 10 recipients were serum CTLA4Ig positive with their concentrations being 14 ng/ml and 31 ng/ml respectively. The average time of maintaining blood glucose normal levels in the group A after pancreatic islet allograft,(14.8±12.3)days,was significantly longer than (3.6±5.1)days of the roup B(P＜0.05),The average survival time in the group A.(24.0±10.8)days (the longest time was 45 days), was significantly longer than(11.8±4.8)days(the longest time was 21 days)of the group B (P＜0.01). Conclusions The muscle cells and pancreatic islets of the recipient rats were transfected with CTLA4Ig cDNA packaged with lipofectin.CTLA4Ig cDNA could be expressed in the recipient tissues. The expressed product could obviously prolong the survival time of the pancreatic islet allograft and the recipient rats and partly inhibit the cellular immunity to prevent rejection.