CAJ | 학술논문

目的探讨CXCR4和CD133mRNA在胃腺癌原发灶中的表达及其与各临床病理参数之间的关系.分析CXCR4和CD133mRNA二者间的相关关系.方法分析50例胃腺癌原发灶和癌旁胃黏膜组织行免疫组化法定位检测CXCR4和CD133 mRNA的相对表达率,并用半定量RT- PCR法测定CXCR4和CD133mRNA的相对灰度值,分析上述两者间的相互关系(Fisher精确概率法).分析CXCR4和CD133mRNA与临床病理因素之间的联系(Spearman相关分析).结果 CXCR4和CD133mRNA在胃腺原发灶中的阳性表达率分别为76.0％和66.0％,显著高于癌旁胃黏膜组织中的16.0％ (P =0.000)和10.0％ (P =0.000).肿瘤直径越大、TNM分期越晚及淋巴管浸润存在,则CXCR4mRNA 灰度值就越高(P＜0.05).肿瘤较大,TNM分期较晚者,CD133mRNA灰度值越高(P＜0.05).CXCR4 和CD133mRNA相对灰度值变化呈正相关(r=0.453,P＜0.01).结论 CXCR4和CD133mRNA的高表达与胃癌的肿瘤直径、TNM分期及淋巴管浸润有关.CD133mRNA相对灰度值随CXCR4mRNA相对灰度值增加而增加.
목적 탐토CXCR4화CD133mRNA재위선암원발조중적표체급기여각림상병리삼수지간적관계.분석CXCR4화CD133mRNA이자간적상관관계.방법 분석50례위선암원발조화암방위점막조직행면역조화법정위검측CXCR4화CD133 mRNA적상대표체솔,병용반정량RT- PCR법측정CXCR4화CD133mRNA적상대회도치,분석상술량자간적상호관계(Fisher정학개솔법).분석CXCR4화CD133mRNA여림상병리인소지간적련계(Spearman상관분석).결과 CXCR4화CD133mRNA재위선원발조중적양성표체솔분별위76.0％화66.0％,현저고우암방위점막조직중적16.0％ (P =0.000)화10.0％ (P =0.000).종류직경월대、TNM분기월만급림파관침윤존재,칙CXCR4mRNA 회도치취월고(P＜0.05).종류교대,TNM분기교만자,CD133mRNA회도치월고(P＜0.05).CXCR4 화CD133mRNA상대회도치변화정정상관(r=0.453,P＜0.01).결론 CXCR4화CD133mRNA적고표체여위암적종류직경、TNM분기급림파관침윤유관.CD133mRNA상대회도치수CXCR4mRNA상대회도치증가이증가.
Objective To investigate the expression of CXCR4 and CD133mRNA in primary lesion of primary gastric adenocarcinoma and the relation with clinicopathological features,and to explore the correlation of CXCR4 and CD133.Methods The primary lesion of primary gastric adenocarcinoma and normal tissues adjacent to gastric cancer were obtained from 50 patients.The diction of CXCR4 and CD133 protein expression was detected by the immunohistochemical staining,and the relative gray scale values of CXCR4 and CD133mRNA by semi-quantitative RT-PCR (Fisher' s exact probability method).Their relationship with clinicopathological features was also investigated ( Spearman relation analysis).Results The positive rates of CXCR4 and CD133 protein in gastric cancers were 76.0％ and 66.0％ respectively,which were significantly higher than that in normal tissues adjacent to gastric cancer ( 16.0％ and 10％ ; P =0.000,P =0.000).The increment of relative gray scale values of CXCR4mRNA was associated with the larger tumor diameter,the later TNM stage and the occurrence of lymphatic metastasis( P ＜ 0.05 ).And the larger diameter of tumor,the later TNM stage were associated with the higher relative gray scale values of CD133mRNA (P ＜0.05).The levels of the relative gray scale values of CXCR4 mRNA and CD133mRNA were positively related(r =0.453,P ＜ 0.01 ).Conclusions The higher expression of CXCR4 and CD133mRNA correlates with tumour diameter,TNM stage and lymphatic vessel invasion. The relative gray scale values of CD133mRNA increase with the increment of the relative gray scale values of CXCR4.