CAJ | 학술논문

目的探讨bcr-abl mRNA在慢性粒细胞白血病(CML)诊断、治疗及微小残留病变监测中的意义.方法采用实时定量聚合酶链反应(real-time PCR)方法检测324例CML患者518份标本的bcr-abl mRNA表达情况.结果 CML慢性期、加速期和急变期患者的bcr-abl mRNA定量结果逐渐增高,分别为12.6%、25.4%和57.2%(P<0.05).异基因造血干细胞移植后的bcr-abl mRNA定量结果随时间延长逐渐降低,一般在移植6个月后转阴,服用伊马替尼(商品名:格列卫)与异基因造血干细胞移植病程相似,但融合基因转阴所用时间较长.结论 real-time PCR检测bcr-abl mRNA对于诊断、评价疗效、监测微小残留病变以及预测疾病进展具有重要的临床应用价值.
목적 탐토bcr-abl mRNA재만성립세포백혈병(CML)진단、치료급미소잔류병변감측중적의의.방법 채용실시정량취합매련반응(real-time PCR)방법검측324례CML환자518빈표본적bcr-abl mRNA표체정황.결과 CML만성기、가속기화급변기환자적bcr-abl mRNA정량결과축점증고,분별위12.6%、25.4%화57.2%(P<0.05).이기인조혈간세포이식후적bcr-abl mRNA정량결과수시간연장축점강저,일반재이식6개월후전음,복용이마체니(상품명:격렬위)여이기인조혈간세포이식병정상사,단융합기인전음소용시간교장.결론 real-time PCR검측bcr-abl mRNA대우진단、평개료효、감측미소잔류병변이급예측질병진전구유중요적림상응용개치.
Objective To evaluate significance of the quantification of bcr-abl mRNA in diagnosis and therapy of chronic myeloid leukemia (CML),essentiality significance for monitoring minimal residual disease. Methods Bcr-abl mRNA of 518 CML patients were detected using real-time PCR. Results Expression of bcr-abl mRNA was gradually increased among blastic phase (BP) (12.6 %),accelerated phase (AP) (25.4 %) and chronic phase (CP) (57.2 %) (P<0.05). Quantification of bcr-abl mRNA was cut down gradually after allotransplantation in the patients and becomes normal after treatment for 6 months. But quantification of bcr-abl mRNA inpatients treated with imatinib mesylate became normal after 12 months. Conclusion Real-time PCR was reliable and can be used for diagnosis,monitoring the treatment outcome,detecting the minimal residual disease,and predicting blast crisis.