中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
11期
961-963
,共3页
帕金森病%改构体酸性成纤维细胞生长因子%羟基多巴胺%大鼠%旋转行为
帕金森病%改構體痠性成纖維細胞生長因子%羥基多巴胺%大鼠%鏇轉行為
파금삼병%개구체산성성섬유세포생장인자%간기다파알%대서%선전행위
Parkinson's disease%Nonmitogenic aFGF%Hydroxydopamine%Rat%Rotary behavior
目的 观察改构体酸性成纤维细胞生长因子(aFGF)对帕金森病大鼠模型行为学的影响.方法 采用SD雄性大鼠72只,随机分为假手术组,PD模型组,PD模型加生理盐水组(PD+NS组),PD模型加改构体aFGF组(PD+aFGF组),每组18只.通过脑内立体定向术,将6-OHDA注入后3组大鼠左侧黑质致密部(substantia nigra pars compacta,SNC)和中脑被盖腹侧区(ventral tegmeatal area,VTA)以建立帕金森病模型,PD+Ns组于术后第1天起每隔2d于右侧侧脑室注射10μl Ns,PD+aFGF组于术后第1天起每隔2d于右侧侧脑室注射10μl改构体aFGF(0.2μg/μl),假手术组只进行假手术处理,分别于术后4d,1,2,3,4周用阿扑吗啡(apomorphine,APO)给大鼠腹腔注射(0.5mg/kg),观察并记录大鼠行为学变化情况.结果 假手术组均未出现旋转行为,PD模型组和PD+Ns组大鼠旋转启动时间逐渐缩短,持续时间逐渐延长,旋转速度逐渐加快,至术后2周旋转行为趋于稳定;在术后2周,PD+aFGF组旋转启动时间[(5.50±1.18)min],较PD模型组[(3.60±1.17)min,P<0.05]和PD+Ns组[(3.10±1.02)min,P<0.05]明显延长;PD+aFGF组旋转持续时间[(25.90±8.80)min],较PD模型组[(55.40±10.14)min,P<0.05]和PD+Ns组[(53.90±12.27)min,P<0.05]明显缩短;PD+aFGF组旋转平均速度[(6.52±1.34)r/min],较PD模型组[(12.90±2.21)r/min,P<0.05]和PD+Ns组[(11.80±3.65)r/min,P<0.05]明显减慢.术后3周、4周,大鼠旋转行为各项指标统计学分析结果 与术后2周相同.结论 PD模型大鼠具有明显得行为学改变,改构体aFGF能明显改善大鼠的旋转行为.
目的 觀察改構體痠性成纖維細胞生長因子(aFGF)對帕金森病大鼠模型行為學的影響.方法 採用SD雄性大鼠72隻,隨機分為假手術組,PD模型組,PD模型加生理鹽水組(PD+NS組),PD模型加改構體aFGF組(PD+aFGF組),每組18隻.通過腦內立體定嚮術,將6-OHDA註入後3組大鼠左側黑質緻密部(substantia nigra pars compacta,SNC)和中腦被蓋腹側區(ventral tegmeatal area,VTA)以建立帕金森病模型,PD+Ns組于術後第1天起每隔2d于右側側腦室註射10μl Ns,PD+aFGF組于術後第1天起每隔2d于右側側腦室註射10μl改構體aFGF(0.2μg/μl),假手術組隻進行假手術處理,分彆于術後4d,1,2,3,4週用阿撲嗎啡(apomorphine,APO)給大鼠腹腔註射(0.5mg/kg),觀察併記錄大鼠行為學變化情況.結果 假手術組均未齣現鏇轉行為,PD模型組和PD+Ns組大鼠鏇轉啟動時間逐漸縮短,持續時間逐漸延長,鏇轉速度逐漸加快,至術後2週鏇轉行為趨于穩定;在術後2週,PD+aFGF組鏇轉啟動時間[(5.50±1.18)min],較PD模型組[(3.60±1.17)min,P<0.05]和PD+Ns組[(3.10±1.02)min,P<0.05]明顯延長;PD+aFGF組鏇轉持續時間[(25.90±8.80)min],較PD模型組[(55.40±10.14)min,P<0.05]和PD+Ns組[(53.90±12.27)min,P<0.05]明顯縮短;PD+aFGF組鏇轉平均速度[(6.52±1.34)r/min],較PD模型組[(12.90±2.21)r/min,P<0.05]和PD+Ns組[(11.80±3.65)r/min,P<0.05]明顯減慢.術後3週、4週,大鼠鏇轉行為各項指標統計學分析結果 與術後2週相同.結論 PD模型大鼠具有明顯得行為學改變,改構體aFGF能明顯改善大鼠的鏇轉行為.
목적 관찰개구체산성성섬유세포생장인자(aFGF)대파금삼병대서모형행위학적영향.방법 채용SD웅성대서72지,수궤분위가수술조,PD모형조,PD모형가생리염수조(PD+NS조),PD모형가개구체aFGF조(PD+aFGF조),매조18지.통과뇌내입체정향술,장6-OHDA주입후3조대서좌측흑질치밀부(substantia nigra pars compacta,SNC)화중뇌피개복측구(ventral tegmeatal area,VTA)이건립파금삼병모형,PD+Ns조우술후제1천기매격2d우우측측뇌실주사10μl Ns,PD+aFGF조우술후제1천기매격2d우우측측뇌실주사10μl개구체aFGF(0.2μg/μl),가수술조지진행가수술처리,분별우술후4d,1,2,3,4주용아복마배(apomorphine,APO)급대서복강주사(0.5mg/kg),관찰병기록대서행위학변화정황.결과 가수술조균미출현선전행위,PD모형조화PD+Ns조대서선전계동시간축점축단,지속시간축점연장,선전속도축점가쾌,지술후2주선전행위추우은정;재술후2주,PD+aFGF조선전계동시간[(5.50±1.18)min],교PD모형조[(3.60±1.17)min,P<0.05]화PD+Ns조[(3.10±1.02)min,P<0.05]명현연장;PD+aFGF조선전지속시간[(25.90±8.80)min],교PD모형조[(55.40±10.14)min,P<0.05]화PD+Ns조[(53.90±12.27)min,P<0.05]명현축단;PD+aFGF조선전평균속도[(6.52±1.34)r/min],교PD모형조[(12.90±2.21)r/min,P<0.05]화PD+Ns조[(11.80±3.65)r/min,P<0.05]명현감만.술후3주、4주,대서선전행위각항지표통계학분석결과 여술후2주상동.결론 PD모형대서구유명현득행위학개변,개구체aFGF능명현개선대서적선전행위.
Objective To investigate the effects of nonmitogenic aFGF on the behavioral changes of Parkinson's disease rat model.Methods Seventy-two male SD rats were divided randomly into 4 groups:Shame group,PD group,PD + NS group and PD + aFGF group,and stereotaxic injection of 6-OHDA into the left substantia nigra pars compaeta (SNC) and ventral tegmental area (VTA) of the rats in the latter three groups to develop PD rat model.In the PD + NS group,injected 10 μl NS into the right lateral ventricle of the rats at 2 days interval since 1 day after the injection of 6-OHDA.In the PD +aFGF group,injected 10 μl(0.2 μg/μl) nonmitogenic aFGF into the right lateral ventricle of the rats at 2 days interval since 1 day after the injection of 6-OHDA.In the Shame group,the rats were injected natural saline(NS) instead of 6-OHDA into the left SNC and VTA.Observed and recorded the behavioral changes of the rats at 4 days,1,2,3,4 weeks after the injection of 6-OHDA.Results No rotary behaviors were observed in the Shame group.In PD group and PD + NS group,the start time of PD rats shortened gradually,the continuous time lengthened,and the rotation rate speeded by degrees.The rotary behavior became invariable until 2 weeks after injection.2 weeks later,in PD + aFGF group,the start time [(5.50±1.18)min] was longer than the PD group[(3.60±1.17)min,P<0.05] and the PD+NS group[(3.10±1.02)min,P<0.05] ;the continuous time [(25.90±8.80)min] was shorter than the PD group [(55.40±10.14) min,P<0.05] and the PD + NS group[(53.90±12.27)min,P<0.05] ;the rotation rate [(6.52±1.34)r/min] was slower than the PD group[(12.90±2.21)r/min,P<0.05] and the PD + NS group [(11.80±3.65)r/min,P<0.05].Compared the rotary behaviors between the three groups,the same statistical results as 2 weeks later can be found at 3 or 4 weeks later.Conclusion PD rats have serious behavioral changes,and the nonmitogenic aFGF may better the rotary behavior of the PD rats.
