中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
8期
579-582
,共4页
张娆%巫丰宏%周旋%彭开良
張嬈%巫豐宏%週鏇%彭開良
장요%무봉굉%주선%팽개량
过氯酸铵%甲状腺素类%睾酮%精子能动性
過氯痠銨%甲狀腺素類%睪酮%精子能動性
과록산안%갑상선소류%고동%정자능동성
Ammonium perchlorate%Thyroid hormone%Testosterone%Sperm motility
目的 研究过氯酸铵(ammonium perchlorate,AP)对雄性大鼠甲状腺激素及睾丸的影响.方法 雄性SD大鼠20只,随机分为对照组、AP低、中、高剂量组,染毒剂量分别为0、130、260、520mg·kg-1·d-1,以大鼠限量饮水方式连续染毒80 d.检测大鼠血清中甲状腺激素、血清睾酮(T)水平、精子活力,并观察睾丸组织的病理变化.结果 AP染毒3周至80 d,中、高剂量组大鼠体重增长明显低于对照组,差异有统计学意义(P＜0.05,P＜0.01),各组大鼠甲状腺湿重及甲状腺脏器系数均高于对照组,差异有统计学意义(P＜0.05,P＜0.01).高剂量组大鼠睾丸脏器系数明显高于对照组,差异有统计学意义(P＜0.01).各剂量组游离甲状腺素(FT4)水平随染毒剂量的增加均明显降低,与对照组比较,差异有统计学意义(P＜0.05),而游离三碘甲状腺原氨酸(FT3)水平无明显变化.中、高剂量组促甲状腺激素(TSH)均明显低于对照组,差异有统计学意义(P＜0.01).中、高剂量组A、B级精子百分比[A∶(12.3%±2.52%)、(14.8%±5.93%),B∶(17.7%±4.63%)、(15.8%±2.28%)]明显低于对照组(27.8%±8.70%),差异有统计学意义(P＜0.01),而D级精子百分比随染毒剂量的增加而逐渐增加,中、高剂量组D级精子百分比[(38.0%±3.61%),(40.0%±8.99%)]明显高于对照组(17.0%±5.00%),差异有统计学意义(P＜0.01).各组大鼠血清中血清T水平没有明显改变.结论 AP可对大鼠甲状腺激素水平产生影响,能引起FT4下降;对雄性生殖系统的毒作用主要是使精子活力发生减退性改变.
目的 研究過氯痠銨(ammonium perchlorate,AP)對雄性大鼠甲狀腺激素及睪汍的影響.方法 雄性SD大鼠20隻,隨機分為對照組、AP低、中、高劑量組,染毒劑量分彆為0、130、260、520mg·kg-1·d-1,以大鼠限量飲水方式連續染毒80 d.檢測大鼠血清中甲狀腺激素、血清睪酮(T)水平、精子活力,併觀察睪汍組織的病理變化.結果 AP染毒3週至80 d,中、高劑量組大鼠體重增長明顯低于對照組,差異有統計學意義(P＜0.05,P＜0.01),各組大鼠甲狀腺濕重及甲狀腺髒器繫數均高于對照組,差異有統計學意義(P＜0.05,P＜0.01).高劑量組大鼠睪汍髒器繫數明顯高于對照組,差異有統計學意義(P＜0.01).各劑量組遊離甲狀腺素(FT4)水平隨染毒劑量的增加均明顯降低,與對照組比較,差異有統計學意義(P＜0.05),而遊離三碘甲狀腺原氨痠(FT3)水平無明顯變化.中、高劑量組促甲狀腺激素(TSH)均明顯低于對照組,差異有統計學意義(P＜0.01).中、高劑量組A、B級精子百分比[A∶(12.3%±2.52%)、(14.8%±5.93%),B∶(17.7%±4.63%)、(15.8%±2.28%)]明顯低于對照組(27.8%±8.70%),差異有統計學意義(P＜0.01),而D級精子百分比隨染毒劑量的增加而逐漸增加,中、高劑量組D級精子百分比[(38.0%±3.61%),(40.0%±8.99%)]明顯高于對照組(17.0%±5.00%),差異有統計學意義(P＜0.01).各組大鼠血清中血清T水平沒有明顯改變.結論 AP可對大鼠甲狀腺激素水平產生影響,能引起FT4下降;對雄性生殖繫統的毒作用主要是使精子活力髮生減退性改變.
목적 연구과록산안(ammonium perchlorate,AP)대웅성대서갑상선격소급고환적영향.방법 웅성SD대서20지,수궤분위대조조、AP저、중、고제량조,염독제량분별위0、130、260、520mg·kg-1·d-1,이대서한량음수방식련속염독80 d.검측대서혈청중갑상선격소、혈청고동(T)수평、정자활력,병관찰고환조직적병리변화.결과 AP염독3주지80 d,중、고제량조대서체중증장명현저우대조조,차이유통계학의의(P＜0.05,P＜0.01),각조대서갑상선습중급갑상선장기계수균고우대조조,차이유통계학의의(P＜0.05,P＜0.01).고제량조대서고환장기계수명현고우대조조,차이유통계학의의(P＜0.01).각제량조유리갑상선소(FT4)수평수염독제량적증가균명현강저,여대조조비교,차이유통계학의의(P＜0.05),이유리삼전갑상선원안산(FT3)수평무명현변화.중、고제량조촉갑상선격소(TSH)균명현저우대조조,차이유통계학의의(P＜0.01).중、고제량조A、B급정자백분비[A∶(12.3%±2.52%)、(14.8%±5.93%),B∶(17.7%±4.63%)、(15.8%±2.28%)]명현저우대조조(27.8%±8.70%),차이유통계학의의(P＜0.01),이D급정자백분비수염독제량적증가이축점증가,중、고제량조D급정자백분비[(38.0%±3.61%),(40.0%±8.99%)]명현고우대조조(17.0%±5.00%),차이유통계학의의(P＜0.01).각조대서혈청중혈청T수평몰유명현개변.결론 AP가대대서갑상선격소수평산생영향,능인기FT4하강;대웅성생식계통적독작용주요시사정자활력발생감퇴성개변.
Objective To study the effects of ammonium perchlorate (AP) on the levels of thyroid hormone and the testis function of male rats.Methods Twenty male rats were randomly divided into 4 groups:control group, low, middle and high AP group.The rats were exposed orally to 0, 130, 260 and 520 mg AP/kg a day for 80 days.The levels of thyroid hormone, testosterone in serum and sperm motility were measured and the testis histological change was observed as well.Results The increase of body weight in high AP group was significantly lower than that in the control group (P＜0.01).The organ coefficients of testis and thyroid in high AP group obviously enhanced, as compared with the control group (P＜0.01).The free thyroxin (FT4) levels of serum in all AP treated groups were significantly lower than that of the control group (P＜0.05).There were no differences of serum FT3 levels between all AP groups and control group, while serum TSH levels in middle and high AP groups decreased significantly, as compared with control group (P＜0.01).In terms of sperm motility, the percentage of Grade A and B sperm in middle and high groups were 12.3%±2.52% and 14.8%±5.93%, 17.7%±4.63%, 15.8%±2.28% respectively, which were significantly lower than that (27.8%±8.70%) in control group (P＜0.01 ).The percentage of Grade D sperm in middle and high groups were 38.0%±3.61% and 40.0%±8.99%, respectively, which were significantly higher than that (17.0%±5.00%) in control group (P＜0.01).No difference of serum testosterone level between all AP groups and control group was observed.Conclusion AP can influence the levels of thyroid hormone and reduce the serum FT4 levels in rats.The main toxic effects on male reproductive system may decrease the sperm motility.
