中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
10期
1463-1465
,共3页
郑顺贞%Karin L(o)w%Matthis Wacker%Arno Wiehe%Sylvia Wagner%Klaus Langer%Hagen von Briesen%邹声泉
鄭順貞%Karin L(o)w%Matthis Wacker%Arno Wiehe%Sylvia Wagner%Klaus Langer%Hagen von Briesen%鄒聲泉
정순정%Karin L(o)w%Matthis Wacker%Arno Wiehe%Sylvia Wagner%Klaus Langer%Hagen von Briesen%추성천
光动力治疗%光敏剂%mTHPC%Jurkat细胞
光動力治療%光敏劑%mTHPC%Jurkat細胞
광동력치료%광민제%mTHPC%Jurkat세포
Photodynamic therapy%Photosensitizer%mTHPC%Jurkat cells
目的 观察光敏剂间-四羟基苯二氢卟酚(mTHPC)及其复合物HSA-mTHPC对人T淋巴细胞白血病细胞(Jurkat)的作用,探讨人血白蛋白(HSA)纳米颗粒作为mTHPC载体的可行性.方法 利用流式细胞仪测定Jurkat细胞对mTHPC及HSA-mTHPC的摄取率,通过测定不同浓度mTHPC及HSA-mTHPC孵育Jurkat细胞后的存活率、细胞膜完整性和增殖率,评价两种药物的摄取率和细胞毒性.结果 浓度1.0 mg/L,孵育4、24 h Jurkat细胞的摄取率为:mTHPC单体:98.07%、99.07%;HSA-mTHPG中mTHPC:98.13%、99.19%;HSA单体:2.77%、4.84%.光照后Jurkat细胞活性和增殖率呈显著剂量、时间依赖的下降,mTHPC浓度为1.0 mg/L时,4、24、48 h存活率和增殖率分别为13.27%、5.46%、4.99%和2.68%、0.91%、0.62%,HSA-mTHPC对细胞生长和增殖的抑制与mTHPC单体比较未见下降(P＞0.05).光照后LDH活性呈现显著剂量、时间依赖的上升,mTHPC浓度为1.0mg/L时,4、24、48 h LDH活性分别为:80.99%、99.38%、100.67%.结论 mTHPC是一种杀伤力强的光敏剂型,能有效进入肿瘤细胞,并导致细胞损害.HSA纳米颗粒不影响细胞对mTHPC的摄取,可以作为其载体.
目的 觀察光敏劑間-四羥基苯二氫卟酚(mTHPC)及其複閤物HSA-mTHPC對人T淋巴細胞白血病細胞(Jurkat)的作用,探討人血白蛋白(HSA)納米顆粒作為mTHPC載體的可行性.方法 利用流式細胞儀測定Jurkat細胞對mTHPC及HSA-mTHPC的攝取率,通過測定不同濃度mTHPC及HSA-mTHPC孵育Jurkat細胞後的存活率、細胞膜完整性和增殖率,評價兩種藥物的攝取率和細胞毒性.結果 濃度1.0 mg/L,孵育4、24 h Jurkat細胞的攝取率為:mTHPC單體:98.07%、99.07%;HSA-mTHPG中mTHPC:98.13%、99.19%;HSA單體:2.77%、4.84%.光照後Jurkat細胞活性和增殖率呈顯著劑量、時間依賴的下降,mTHPC濃度為1.0 mg/L時,4、24、48 h存活率和增殖率分彆為13.27%、5.46%、4.99%和2.68%、0.91%、0.62%,HSA-mTHPC對細胞生長和增殖的抑製與mTHPC單體比較未見下降(P＞0.05).光照後LDH活性呈現顯著劑量、時間依賴的上升,mTHPC濃度為1.0mg/L時,4、24、48 h LDH活性分彆為:80.99%、99.38%、100.67%.結論 mTHPC是一種殺傷力彊的光敏劑型,能有效進入腫瘤細胞,併導緻細胞損害.HSA納米顆粒不影響細胞對mTHPC的攝取,可以作為其載體.
목적 관찰광민제간-사간기분이경계분(mTHPC)급기복합물HSA-mTHPC대인T림파세포백혈병세포(Jurkat)적작용,탐토인혈백단백(HSA)납미과립작위mTHPC재체적가행성.방법 이용류식세포의측정Jurkat세포대mTHPC급HSA-mTHPC적섭취솔,통과측정불동농도mTHPC급HSA-mTHPC부육Jurkat세포후적존활솔、세포막완정성화증식솔,평개량충약물적섭취솔화세포독성.결과 농도1.0 mg/L,부육4、24 h Jurkat세포적섭취솔위:mTHPC단체:98.07%、99.07%;HSA-mTHPG중mTHPC:98.13%、99.19%;HSA단체:2.77%、4.84%.광조후Jurkat세포활성화증식솔정현저제량、시간의뢰적하강,mTHPC농도위1.0 mg/L시,4、24、48 h존활솔화증식솔분별위13.27%、5.46%、4.99%화2.68%、0.91%、0.62%,HSA-mTHPC대세포생장화증식적억제여mTHPC단체비교미견하강(P＞0.05).광조후LDH활성정현현저제량、시간의뢰적상승,mTHPC농도위1.0mg/L시,4、24、48 h LDH활성분별위:80.99%、99.38%、100.67%.결론 mTHPC시일충살상력강적광민제형,능유효진입종류세포,병도치세포손해.HSA납미과립불영향세포대mTHPC적섭취,가이작위기재체.
Objective To investigate the interaction and cytotoxicity of the photosensitizer mTHPC [5, 10, 15, 20-tetrakis (3-hydroxyphenyl) chlorine]and the complex human serum albumin (HSA)-mTHPC in Jurkat cells. Methods Jurkat cells were incubated with different concentrations of mTHPC and HSA-mTHPC for4, 24 and 48 h, followed by illumination with 652 nm for 10 min with 10 mW/cm2. The cellular interaction of free mTHPC and HSA-mTHPC was measured by using flow cytometry. The cytotoxicity was tested using standard toxicity assays (the WST-l-assay for the cell viability, the LDH-assay for the membrane integrity, and the BrdU-assay for the proliferation). Results After incubation with 1.0 mg/L mTHPC for 4 and 24 h, 98.07% and 99.07% of the cells were positive for the photosensitizer. The interaction of HSA without drug with the cells was low. The uptake of mTHPC that was compounded to HSA was as high as of free mTHPC (4 h: 98.13%; 24 h: 99.19%). After illumination, the cell viability was decreased to 13.27%, 5.46% and 4.99% after incubation with 1.0 mg/L mTHPC 4, 24 and 48 h, respectively. The LDH leakage was increased up to 100% even at low concentrations of mTHPC. The cell proliferation was also decreased in a concentration-and time-dependent manner. After incubation with 1.0 mg/L mTHPC for 48 h and subsequent illumination, only marginal proliferation could be detected (0.62% ). Conclusion Both mTHPC and HSA-mTHPC are photosensitizer formulations with potent anti-tumor effects, which can enter tumor cells effectively and cause cell death. HSA nanoparticles can be used as mTHPC carriers.
