中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2009年
5期
351-355
,共5页
徐刚%王槐富%何刚%谢可
徐剛%王槐富%何剛%謝可
서강%왕괴부%하강%사가
鼻肿瘤%非霍奇金淋巴瘤%预后%p53蛋白%潜伏膜蛋白1
鼻腫瘤%非霍奇金淋巴瘤%預後%p53蛋白%潛伏膜蛋白1
비종류%비곽기금림파류%예후%p53단백%잠복막단백1
Nasal neoplasms%non-Hodgkin's lymphoma%Prognosis%p53 protein%Latent membrane protein 1
目的 探讨鼠双微体2(mdm2)、p53、p21基因和潜伏膜蛋白1(LMP-1)在鼻腔NK/T细胞淋巴瘤(NKTL)中的表达及相互作用关系,以及其与NKTL临床分期和预后的关系.方法 收集62例NKTL患者的临床病理资料,并进行随访.取62例NKTL组织构建组织芯片,采用免疫组化SP法检测mdm2、pS3、p21及LMP-1蛋白的表达情况;应用原位杂交法检测EBER1/2的表达.结果 mdm2、p53、p21和LMP-1蛋白在NKTL中的阳性表达率分别为61.3%、79.0%、58.1%和48.4%,EBER1/2的阳性表达率为90.3%.随着肿瘤临床分期的进展,mdm2、p53和p21蛋白的阳性表达率逐渐升高,并与临床分期相关(均P<0.05).mdm2、p53和p21蛋白的表达呈正相关(P<0.05),其阴性表达组患者的预后均好于阳性表达组(均P<0.05).LMP-1蛋白的表达与临床分期和预后无关(P>0.05).p53蛋白的表达水平是NKTL的独立预后因素.结论 mdm2、p53、p21的蛋白表达与NKTL的发生和发展密切相关,可作为评估NKTL生物学行为的良好指标.p53蛋白的表达水平是NKTL的独立预后因素.
目的 探討鼠雙微體2(mdm2)、p53、p21基因和潛伏膜蛋白1(LMP-1)在鼻腔NK/T細胞淋巴瘤(NKTL)中的錶達及相互作用關繫,以及其與NKTL臨床分期和預後的關繫.方法 收集62例NKTL患者的臨床病理資料,併進行隨訪.取62例NKTL組織構建組織芯片,採用免疫組化SP法檢測mdm2、pS3、p21及LMP-1蛋白的錶達情況;應用原位雜交法檢測EBER1/2的錶達.結果 mdm2、p53、p21和LMP-1蛋白在NKTL中的暘性錶達率分彆為61.3%、79.0%、58.1%和48.4%,EBER1/2的暘性錶達率為90.3%.隨著腫瘤臨床分期的進展,mdm2、p53和p21蛋白的暘性錶達率逐漸升高,併與臨床分期相關(均P<0.05).mdm2、p53和p21蛋白的錶達呈正相關(P<0.05),其陰性錶達組患者的預後均好于暘性錶達組(均P<0.05).LMP-1蛋白的錶達與臨床分期和預後無關(P>0.05).p53蛋白的錶達水平是NKTL的獨立預後因素.結論 mdm2、p53、p21的蛋白錶達與NKTL的髮生和髮展密切相關,可作為評估NKTL生物學行為的良好指標.p53蛋白的錶達水平是NKTL的獨立預後因素.
목적 탐토서쌍미체2(mdm2)、p53、p21기인화잠복막단백1(LMP-1)재비강NK/T세포림파류(NKTL)중적표체급상호작용관계,이급기여NKTL림상분기화예후적관계.방법 수집62례NKTL환자적림상병리자료,병진행수방.취62례NKTL조직구건조직심편,채용면역조화SP법검측mdm2、pS3、p21급LMP-1단백적표체정황;응용원위잡교법검측EBER1/2적표체.결과 mdm2、p53、p21화LMP-1단백재NKTL중적양성표체솔분별위61.3%、79.0%、58.1%화48.4%,EBER1/2적양성표체솔위90.3%.수착종류림상분기적진전,mdm2、p53화p21단백적양성표체솔축점승고,병여림상분기상관(균P<0.05).mdm2、p53화p21단백적표체정정상관(P<0.05),기음성표체조환자적예후균호우양성표체조(균P<0.05).LMP-1단백적표체여림상분기화예후무관(P>0.05).p53단백적표체수평시NKTL적독립예후인소.결론 mdm2、p53、p21적단백표체여NKTL적발생화발전밀절상관,가작위평고NKTL생물학행위적량호지표.p53단백적표체수평시NKTL적독립예후인소.
Objective To investigate the expression of murine double minute 2 (mdm2),p53, p21 and latent membrane protein 1 (LMP-1) in nasal NK/T-cell lymphoma (NKTL) and analyze their relationship with the clinical stage and prognosis. Methods The clinicopathological and follow-up data of 62 patients with NKTL proven by pathology were collected. Paraffin-embedded tissue sections were examined for mdm2, p53, p21 and LMP-1 proteins by tissue microarray technique and immunohistochemistry. In situ hybridization was used to detect EBER1/2. Results The positive expression rates of mdm2, p53, p21and LMP-1 proteins in NKTL were 61.3%, 79.0%, 58. 1% and 48.4%, respectively, and EBER1/2 was 90.3%. The positive expression rates of mdm2 in Ann Arbor stage Ⅰ , Ⅱ, Ⅲ and Ⅳ NKTL were 43.5%, 62.5%, 73.3% and 87.5%, respectively; p53 were 69.6%, 75.0%, 86.7% and 100.0% ; p21 were 47.8%, 56.3%, 60.0% and 87.5% ; while those of LMP-1 were 60.9%, 50.0%, 26.7% and 50.0%, respectively. With the progression of tumor, the positive expression rates of mdm2, p53 and p21 proteins gradually increased. There were statistically significant differences between them (P < 0. 05). There were statistically positive correlations among those three genes (P < 0. 05). The prognosis of the positive expression group of these three genes was worse than that of negative expression group (P < 0. 05). No statistically significant difference was observed between the expression of LMP-1 protein and the clinical stage or prognosis (P > 0. 05). The expression of p53 protein was an independent prognostic factor. Condusion The expression of mdm2, p53 and p21 proteins is closely related with the pathogenesis and progression of NKTL. They are good markers for judgement of the biological behavior of NKTL. The expression of p53 protein is an independent prognostic factor. Though no significant relationship was found between the expression of LMP-1 protein and the clinical stage or prognosis, it may play some role in tumor progression.
