中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2010年
11期
825-829
,共5页
艾婷%王宁%李围围%宋丽萍
艾婷%王寧%李圍圍%宋麗萍
애정%왕저%리위위%송려평
癌,非小细胞肺%表皮生长因子受体%肝细胞生长因子受体%实时定量聚合酶链反应%免疫组织化学
癌,非小細胞肺%錶皮生長因子受體%肝細胞生長因子受體%實時定量聚閤酶鏈反應%免疫組織化學
암,비소세포폐%표피생장인자수체%간세포생장인자수체%실시정량취합매련반응%면역조직화학
Carcinoma,non-small cell lung%Epidermal growth factor receptor%c-Met%Real-time PCR%Immunohistochemistry
目的 探讨非小细胞肺癌(NSCLC)组织中表皮生长因子受体(EGFR)和肝细胞生长因子受体(c-Met)基因和蛋白的表达情况,及其与NSCLC临床病理特征和预后的关系.方法 采用免疫组化法检测61例NSCLC组织中EGFR和c-Met蛋白的表达情况,采用实时荧光定量PCR法检测EGFR和c-Met DNA的相对拷贝数.结果 61例NSCLC组织中,EGFR和c-Met蛋白的阳性表达率分别为77.0%和57.4%.EGFR和c-Met蛋白在高、中分化NSCLC组织中的阳性表达率分别为61.5%和38.5%,均明显低于低分化的肿瘤组织(均P<0.05).EGFR和c-Met DNA在吸烟患者中的相对拷贝数分别为0.22±0.22和0.20±0.21,均明显高于不吸烟患者(均P<0.05);在腺癌中的相对拷贝数分别为0.24±0.26和0.23±0.25,均明显高于鳞癌患者(均P<0.05).EGFR蛋白与c-Met蛋白表达、EGFR DNA相对拷贝数与c-Met DNA相对拷贝数、EGFR蛋白表达与EGFR DNA相对拷贝数之间均具有显著的相关性(均P<0.05),但c-Met蛋白表达与c-Met DNA相对拷贝数之间没有相关性(P=0.259).EGFR DNA低拷贝组和高拷贝组患者的术后中位生存时间分别为48和36个月,差异有统计学意义(P=0.039);c-Met DNA低拷贝组和高拷贝组患者的术后中位生存时间分别为44和31个月,差异亦有统计学意义(P=0.022).结论 EGFR和c-Met的蛋白表达与NSCLC的分化程度有关;EGFR和c-Met DNA的相对拷贝数与NSCLC患者的吸烟史以及病理类型有关,有助于预测NSCLC患者的预后.
目的 探討非小細胞肺癌(NSCLC)組織中錶皮生長因子受體(EGFR)和肝細胞生長因子受體(c-Met)基因和蛋白的錶達情況,及其與NSCLC臨床病理特徵和預後的關繫.方法 採用免疫組化法檢測61例NSCLC組織中EGFR和c-Met蛋白的錶達情況,採用實時熒光定量PCR法檢測EGFR和c-Met DNA的相對拷貝數.結果 61例NSCLC組織中,EGFR和c-Met蛋白的暘性錶達率分彆為77.0%和57.4%.EGFR和c-Met蛋白在高、中分化NSCLC組織中的暘性錶達率分彆為61.5%和38.5%,均明顯低于低分化的腫瘤組織(均P<0.05).EGFR和c-Met DNA在吸煙患者中的相對拷貝數分彆為0.22±0.22和0.20±0.21,均明顯高于不吸煙患者(均P<0.05);在腺癌中的相對拷貝數分彆為0.24±0.26和0.23±0.25,均明顯高于鱗癌患者(均P<0.05).EGFR蛋白與c-Met蛋白錶達、EGFR DNA相對拷貝數與c-Met DNA相對拷貝數、EGFR蛋白錶達與EGFR DNA相對拷貝數之間均具有顯著的相關性(均P<0.05),但c-Met蛋白錶達與c-Met DNA相對拷貝數之間沒有相關性(P=0.259).EGFR DNA低拷貝組和高拷貝組患者的術後中位生存時間分彆為48和36箇月,差異有統計學意義(P=0.039);c-Met DNA低拷貝組和高拷貝組患者的術後中位生存時間分彆為44和31箇月,差異亦有統計學意義(P=0.022).結論 EGFR和c-Met的蛋白錶達與NSCLC的分化程度有關;EGFR和c-Met DNA的相對拷貝數與NSCLC患者的吸煙史以及病理類型有關,有助于預測NSCLC患者的預後.
목적 탐토비소세포폐암(NSCLC)조직중표피생장인자수체(EGFR)화간세포생장인자수체(c-Met)기인화단백적표체정황,급기여NSCLC림상병리특정화예후적관계.방법 채용면역조화법검측61례NSCLC조직중EGFR화c-Met단백적표체정황,채용실시형광정량PCR법검측EGFR화c-Met DNA적상대고패수.결과 61례NSCLC조직중,EGFR화c-Met단백적양성표체솔분별위77.0%화57.4%.EGFR화c-Met단백재고、중분화NSCLC조직중적양성표체솔분별위61.5%화38.5%,균명현저우저분화적종류조직(균P<0.05).EGFR화c-Met DNA재흡연환자중적상대고패수분별위0.22±0.22화0.20±0.21,균명현고우불흡연환자(균P<0.05);재선암중적상대고패수분별위0.24±0.26화0.23±0.25,균명현고우린암환자(균P<0.05).EGFR단백여c-Met단백표체、EGFR DNA상대고패수여c-Met DNA상대고패수、EGFR단백표체여EGFR DNA상대고패수지간균구유현저적상관성(균P<0.05),단c-Met단백표체여c-Met DNA상대고패수지간몰유상관성(P=0.259).EGFR DNA저고패조화고고패조환자적술후중위생존시간분별위48화36개월,차이유통계학의의(P=0.039);c-Met DNA저고패조화고고패조환자적술후중위생존시간분별위44화31개월,차이역유통계학의의(P=0.022).결론 EGFR화c-Met적단백표체여NSCLC적분화정도유관;EGFR화c-Met DNA적상대고패수여NSCLC환자적흡연사이급병리류형유관,유조우예측NSCLC환자적예후.
Objective To investigate the gene copy number and protein expression of EGFR/cMet, so as to explore the relationship between them and the clinicopathological features and prognosis in nonsmall cell lung cancer (NSCLC) patients. Methods The expression of EGFR/c-Met protein was detected by immunohistochemical staining in NSCLC tissues of 61 cases. The level of EGFR/c-Met gene copy number was detected by fluorescent RT-PCR. Results The positive rates of EGFR and c-Met protein expression were 77.5% and 57.4%, respectively. These rates in well/moderately differentiated NSCLC tissues were 61.5% and 38.5%, all significantly lower than the data in poorly differentiated tissues (P <0. 05). The relative EGFR and c-Met gene copy number was 0.22±0.22 and 0.20±0.21 in smokers, respectively, all significantly higher than that in non-smokers ( P < 0. 05). The gene copy number of EGFR and c-Met in adenocarcinoma was 0.24 ± 0.26 and 0.23 ± 0.25, respectively, all higher than the data in squamous cell carcinoma ( P < 0. 05 ). There was a significant correlation in regard to the EGFR and c-Met protein expression, EGFR and c-Met gene copy number, and the protein expression vs. the gene copy number only in EGFR ( P < 0. 05 ). But there was no significant correlation between the c-Met protein expression and gene copy number ( P = 0. 259 ). There was a statistical significance between the postoperational median survival times (MST) of low EGFR gene copy number (48 months) and the high EGFR gene copy number (36 months) patients (P=0.039). Similarly, there was also a significant difference between the MST of the low and high c-Met gene copy number patients (44 and 31 months, P=0.022). Conclusion There is a correlation between the EGFR and c-Met protein expression and the differentiation of NSCLC. The relative gene copy number is correlated with pathologic types and smoking of NSCLC patients, and it can be used in the prediction of prognosis.