CAJ | 학술논문

目的:观察体外培养肺癌细胞bcl-2表达与机体免疫攻击致癌细胞凋亡的关系。方法:把bcl-2表达不同的肺癌细胞株与从肺癌组织中分离出来的肿瘤浸润淋巴细胞(tumorinfiltratinglymphocyte,TIL)共同培养，通过JAM试验观察肺癌细胞凋亡的情况。结果:体外JAM试验中，随着TIL浓度增加，bcl-2阴性的肺癌细胞凋亡也增加，但bcl-2阳性者则无变化。结论:bcl-2表达阳性的肺癌细胞具有抗凋亡特性，能耐受肿瘤浸润淋巴细胞的免疫攻击。
목적:관찰체외배양폐암세포bcl-2표체여궤체면역공격치암세포조망적관계。방법:파bcl-2표체불동적폐암세포주여종폐암조직중분리출래적종류침윤림파세포(tumorinfiltratinglymphocyte,TIL)공동배양，통과JAM시험관찰폐암세포조망적정황。결과:체외JAM시험중，수착TIL농도증가，bcl-2음성적폐암세포조망야증가，단bcl-2양성자칙무변화。결론:bcl-2표체양성적폐암세포구유항조망특성，능내수종류침윤림파세포적면역공격。
Objective: The current study was designed to investigate the relationship between bcl-2 expression and apoptosis of lung cancer cells in vitro. Methods:Lung cancer cell lines with different expression of bcl-2 were cocultured with tumor infiltrating lymphocyte(TIL) isolated from fresh tumor samples,and JAM test was performed to evaluate apoptosis of cancer cells. Immunohistochemistry and TUNEL assay were used to determine bcl-2 expression and apoptosis of tissue sections of lung cancers respectively. Results: As shown in JAM test, apoptosis increased with the elevation of TIL in coculturing assay in bcl-2 negative cancer cell lines, but in one of bcl-2 positive cell lines it remained stable. Conclusions: Lung cancer cells with bcl-2 expression may antagonize apoptosis, which may account for their immune evasion mechanism.