CAJ | 학술논문

目的:观察红景天素、褪黑素对实验性老年痴呆大鼠的防治作用.方法:连续6周腹腔内注射D-半乳糖,双侧海马内注射Aβ1-40造成AD模型.以Y-型迷宫、一次性被动回避实验检测大鼠的学习记忆行为,测定大脑定歧脂褐素含量以及海马线粒体膜流动性、SOD活性、MDA含量,电镜观察海马超微结构.结果:红景天素、褪黑素可显著改善痴呆大鼠的学习记忆障碍,明显逆转海马SOD活性、MDA含量及皮层脂褐素含量相对其正常水平的增高,阻抑海马神经元的变性及凋亡.结论:红景天素、褪黑素可能通过影响自由基水平对实验性老年性痴呆大鼠有一定的防治作用,但两者协同作用不明显.
목적:관찰홍경천소、퇴흑소대실험성노년치태대서적방치작용.방법:련속6주복강내주사D-반유당,쌍측해마내주사Aβ1-40조성AD모형.이Y-형미궁、일차성피동회피실험검측대서적학습기억행위,측정대뇌정기지갈소함량이급해마선립체막류동성、SOD활성、MDA함량,전경관찰해마초미결구.결과:홍경천소、퇴흑소가현저개선치태대서적학습기억장애,명현역전해마SOD활성、MDA함량급피층지갈소함량상대기정상수평적증고,조억해마신경원적변성급조망.결론:홍경천소、퇴흑소가능통과영향자유기수평대실험성노년성치태대서유일정적방치작용,단량자협동작용불명현.
Objective: To study the protective and therapeutic effects of Rhodosin and Melatonin on Alzheimer's disease(AD) rats. Methods: D-galactose was intraperitoneally injected in rats for 6 weeks and β-Amyloid1-40 (Aβ1-40) was injected into bilateral hippocampus to make AD models. Rhodosin and Melatonin were intraperitoneally injected in rats for 4 weeks to determine the protective and therapeutic effects on rats with AD. Y-maze test, and passive avoidance task were used to determine the ability of learning and memory. The content of lipofuscin in the central correx, the viscous coefficient of mitochondrial membrane, the activity of superoxide dismutase and the content of malondialdehyde in both sides of hippocampus were determined. And the apoptosis of hippocampus neurons was determined with transmission electron microscopy(TEM). Results: Melatonin as an antioxidant significantly improved learning and memory deficits in the rats with AD and reduced the increase in SOD, MDA, the viscous coefficient and lipefuscin to their normal levels, and it also showed the protective effects of apoptosis. Rhodosin showed the similar effects. Conclusion: Rhodosin and Melatonin had preventive and therapeutic effects on rats with AD probably by affecting the free radical levels in rats.