CAJ | 학술논문

木瓜蛋白酶催化水解酪蛋白生成小分子氨基酸和肽,剩余底物酪蛋白与三氯乙酸结合形成粒径约为1 740 nm的缔合物微粒.该微粒在360、400、420、470、520 nm处产生5个共振散射峰,其中最强峰位于470nm.在选定条件下,随着木瓜蛋白酶量的增加,体系中剩余酪蛋白浓度降低,470 nm处的共振散射光强度I470nm降低.木瓜蛋白酶的酶活力在0.024～4.8 USP/mL范围内与470 nm处的共振散射光强度降低值△I<470nm呈现良好线性关系,检出限为0.008 3 USP/mL.该共振散射光谱法用于嫩肉粉中木瓜蛋白酶活力测定,结果满意.
목과단백매최화수해락단백생성소분자안기산화태,잉여저물락단백여삼록을산결합형성립경약위1 740 nm적체합물미립.해미립재360、400、420、470、520 nm처산생5개공진산사봉,기중최강봉위우470nm.재선정조건하,수착목과단백매량적증가,체계중잉여락단백농도강저,470 nm처적공진산사광강도I470nm강저.목과단백매적매활력재0.024～4.8 USP/mL범위내여470 nm처적공진산사광강도강저치△I<470nm정현량호선성관계,검출한위0.008 3 USP/mL.해공진산사광보법용우눈육분중목과단백매활력측정,결과만의.
A catalytic resonance scattering spectral method for assay of papain activity has been developed. Itwas based on the catalytic effect of papain on the hydrolysis of casein to form small molecules ,and the caseinassociated with trichloroacetic acid to form association particles in size of about 1 740 nm,which exhibit fiveresonance scattering (RS) peaks at 470,420,400,360,and 520 nm,with the strongest one at 470 nm. Underthe conditions selected,the casein decreased with the addition of papain. The decreased scattering intensity atwith satisfactory results.