CAJ | 학술논문

本研究旨在检测肿瘤抑制基因p16INK4a(inhibitor of cyclin-dependent kinase 4a)在早孕小鼠子宫内膜中的表达规律,探讨p16INK4a在小鼠胚胎着床过程中的作用.采用荧光定量PCR(FQ-PCR)和免疫组织化学方法分别检测未孕小鼠及孕小鼠第2、3、4、5、7天子宫内膜p16INK4a mRNA和蛋白的表达;子宫角注射p16INK4a抗体观察胚泡着床数.FQ-PCR结果显示孕小鼠子宫内膜组织p16INK4amRNA的表达高于未孕小鼠,且随着妊娠天数的增加呈现表达逐渐增强的趋势,到妊娠第5天达到最高,后渐降.免疫组织化学分析显示p16INK4a蛋白在子宫内膜的表达规律与mRNA结果一致.子宫角注射p16INK4a抗体后胚泡着床数明显减少.以上结果提示,P161INK4a在妊娠早期子宫内膜持续表达,可能参与胚泡着床.
본연구지재검측종류억제기인p16INK4a(inhibitor of cyclin-dependent kinase 4a)재조잉소서자궁내막중적표체규률,탐토p16INK4a재소서배태착상과정중적작용.채용형광정량PCR(FQ-PCR)화면역조직화학방법분별검측미잉소서급잉소서제2、3、4、5、7천자궁내막p16INK4a mRNA화단백적표체;자궁각주사p16INK4a항체관찰배포착상수.FQ-PCR결과현시잉소서자궁내막조직p16INK4amRNA적표체고우미잉소서,차수착임신천수적증가정현표체축점증강적추세,도임신제5천체도최고,후점강.면역조직화학분석현시p16INK4a단백재자궁내막적표체규률여mRNA결과일치.자궁각주사p16INK4a항체후배포착상수명현감소.이상결과제시,P161INK4a재임신조기자궁내막지속표체,가능삼여배포착상.
The expression of tumor suppressor gene p16INK4a in mouse endometrium during early pregnancy and its possible role in blastocyst implantation were investigated in the present study. Real-time fluorescent quantitative PCR (FQ-PCR) and immunohisto-chemistry were applied to detect p16INK4a mRNA and protein expressions in endometrium of un-pregnant and pregnant mice on day 2, 3, 4, 5, 7, respectively. In addition, p16INK4a antibody was injected into the horns of uteri in pregnant mice on day 3 and its effect during blastocyst implantation was detected in vivo. The higher expressions of p16INK4a mRNA and protein were observed in pregnant mice compared with that in un-pregnant mice, with a steady increase from day 2 to day 5 and reaching the maximal level on day 5 of pregnancy and then decreasing, p16INK4a antibody decreased the number of implanted blastocysts compared with that of saline-injected group. The results suggest that p16INK4a may be associated with apoptosis of luminal epithelial cells and decidual cells, coordinating decidualization of endometrium and invasion of trophoblastic cells. Thus, we presume that p16INK4a participates in the process of blastocyst implantation in mice.