CAJ | 학술논문

目的:观察(R,R)-XY-10和(S,S)-XY-10对视网膜色素上皮细胞的增生作用,并且进一步的研究其作用机制.但(R,R)-XY-10和(S,S)-XY-10对人脐静脉内皮细胞并无增生的作用.方法:通过人视网膜色素上皮细胞(ARPE-19)和人脐静脉内皮细胞(HUVECs)研究(R,R)- XY-10和(S,S)-XY-10对视网膜色素上皮细胞的增生作用,并且采用ERK、KT、PI3K、蛋白激酶C(PKC)和一氧化氮合酶(NOS)抑制剂来研究其作用机制.结果:(R,R)-XY-10和(S,S)-XY-10促进了ARPE-19细胞的增生,并具有剂量依赖性,但是对HUVECs细胞没有影响.如果同时加入增生抑制剂H7 (5μmol/L)、金丝桃素(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH-5(10μmol/L)和L-NAME (100μmol/L),则给予H7、金丝桃素、PD98059和LY294002各组的增生作用受到了抑制,而给予SH-5和L-NAME两组的增生作用没有影响.结论:(R,R)-XY-10和(S,S)-XY-10能够诱导ARPE-19细胞增生,其作用可能是通过MAPK和PI3K的途径来发挥该作用.因此,(R,R)-XY-10和(S,S)-XY-10能通过修复损伤的RPE细胞来治疗老年性黄斑变性.
목적:관찰(R,R)-XY-10화(S,S)-XY-10대시망막색소상피세포적증생작용,병차진일보적연구기작용궤제.단(R,R)-XY-10화(S,S)-XY-10대인제정맥내피세포병무증생적작용.방법:통과인시망막색소상피세포(ARPE-19)화인제정맥내피세포(HUVECs)연구(R,R)- XY-10화(S,S)-XY-10대시망막색소상피세포적증생작용,병차채용ERK、KT、PI3K、단백격매C(PKC)화일양화담합매(NOS)억제제래연구기작용궤제.결과:(R,R)-XY-10화(S,S)-XY-10촉진료ARPE-19세포적증생,병구유제량의뢰성,단시대HUVECs세포몰유영향.여과동시가입증생억제제H7 (5μmol/L)、금사도소(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH-5(10μmol/L)화L-NAME (100μmol/L),칙급여H7、금사도소、PD98059화LY294002각조적증생작용수도료억제,이급여SH-5화L-NAME량조적증생작용몰유영향.결론:(R,R)-XY-10화(S,S)-XY-10능구유도ARPE-19세포증생,기작용가능시통과MAPK화PI3K적도경래발휘해작용.인차,(R,R)-XY-10화(S,S)-XY-10능통과수복손상적RPE세포래치료노년성황반변성.
AIM: To investigate the mechanism of proliferation effect induced by (R,R)-XY-10 and (S,S)-XY-10 on retinal pigmented epithelial cells(ARPE-19).METHODS: Human retinal pigmented epithelial cells(ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R,R)-XY-10 and (S,S)-XY-10 on cell growth,and their mechanisms of proliferative action by using ERK、 AKT、PI3K、Protein kinase C (PKC)and Nitric oxide synthase (NOS) inhibitors.RESULTS: (R,R)-XY-10 and (S,S)-XY-10 dose-dependently increased ARPE-19 cell proliferation,but not on HUVECs. When treated with proliferative inhibitors,H7(5μmol/L)、hypericin(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH-5 (10μmol/L) and L-NAME (100μmol/L),the proliferative effect was reduced by H7、hypericin、PD98059 and LY294002,but not by SH-5 and L-NAME.CONCLUSION: (R,R)-XY-10 and (S,S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway. KEYWORDS: age-related macular degeneration; (R,R)-XY-10; (S,S)-XY-10; ARPE-19 cells; human umbilical vein endothelial cells; proliferation