CAJ | 학술논문

[目的]提取"顽拗"植物春石斛的基因组DNA,并对其进行质量检测.[方法]采用普通CTAB法、试剂盒法和改良CTAB法提取10个春石斛品种的基因组DNA,然后分别用紫外分光光度计法、琼脂糖凝胶电泳法和荧光AFLP法对3种方法所提取的基因组DNA质量进行检验.[结果]改良CTAB法所提取春石斛基因组DNA得率较高,基本排除了春石斛体内多糖等次生代谢物质对基因组DNA质量的干扰,完全能满足AFLP等分子生物学试验对DNA质量的要求;其他2种方法所提取的基因组DNA则不能满足试验要求.[结论]改良CTAB法为较好的适合春石斛基因组DNA提取的方法.
[목적]제취"완요"식물춘석곡적기인조DNA,병대기진행질량검측.[방법]채용보통CTAB법、시제합법화개량CTAB법제취10개춘석곡품충적기인조DNA,연후분별용자외분광광도계법、경지당응효전영법화형광AFLP법대3충방법소제취적기인조DNA질량진행검험.[결과]개량CTAB법소제취춘석곡기인조DNA득솔교고,기본배제료춘석곡체내다당등차생대사물질대기인조DNA질량적간우,완전능만족AFLP등분자생물학시험대DNA질량적요구;기타2충방법소제취적기인조DNA칙불능만족시험요구.[결론]개량CTAB법위교호적괄합춘석곡기인조DNA제취적방법.
[Objective] The research aimed to extract the genomic DNA from Spring Dendrobium, and examine its quality. [Method] Three different methods, CTAB method, kit method and modified CTAB method, were chose to extract the high quality DNA from the recalcitrant spring Dendrobium. Then UV spectrophotometer method, agarose gel electrophoresis method and fluorescent AFLP method were used to examine the quality of genomic DNA extracted by 3 extraction methods. [Result] The ratios of the genomic DNA extracted by the modified CTAB method were higher, and could meet the require of molecular biological experiment. But the genomic DNA extracted by the other two methods could not meet the require. [Conclusion] The modified CTAB method is good and suitable for extracting the genomic DNA from Spring Dendrobium.