CAJ | 학술논문

逆境条件下植物体内产生并累积活性氧从而破坏植物组织结构与功能,同时植物也可以通过改变活性氧代谢相关酶活性清除活性氧而减轻活性氧伤害以适应环境胁迫.为研究铝胁迫下不同耐铝小麦品种(Triticum aestivum L.)在活性氧代谢上的差异及与小麦耐铝性的关系,本试验选用小麦品种ET8(耐铝型)、ES8(铝敏感型)为试验材料研究了不同耐铝小麦品种活性氧代谢变化上的差异.结果表明,50μmol·L~(-1)铝处理24 h,ET8和ES8活性氧含量显著升高,O_2~(·-)产生速率增幅分别为10.5%和20.4%,H_2O_2含量增幅分别为3.3%和7.6%.ET8和ES8超氧化物歧化酶(SOD)活性增幅分别为11.9%和41.6%,过氧化物酶(POD)活性增幅为51.8%和77.8%,抗坏血酸过氧化物酶(APX)活性增幅为54.4%和29.1%,过氧化氢酶(CAT)活性增幅为32.9%和38.4%,谷胱甘肽还原酶(GR)活性增幅为83.1%和85.5%.虽然抗氧化酶活性增加后会清除一部分活性氧,但活性氧的累积仍然造成了膜脂的过氧化,ET8和ES8丙二醛(MDA)含量分别增加18.2%和50.0%,质膜透性也随着MDA含量的升高而增加,增幅分别为1.25倍和1.36倍.综上所述,不同耐铝品种间活性氧代谢的差异是小麦品种耐铝性差异显著的原因之一.
역경조건하식물체내산생병루적활성양종이파배식물조직결구여공능,동시식물야가이통과개변활성양대사상관매활성청제활성양이감경활성양상해이괄응배경협박.위연구려협박하불동내려소맥품충(Triticum aestivum L.)재활성양대사상적차이급여소맥내려성적관계,본시험선용소맥품충ET8(내려형)、ES8(려민감형)위시험재료연구료불동내려소맥품충활성양대사변화상적차이.결과표명,50μmol·L~(-1)려처리24 h,ET8화ES8활성양함량현저승고,O_2~(·-)산생속솔증폭분별위10.5%화20.4%,H_2O_2함량증폭분별위3.3%화7.6%.ET8화ES8초양화물기화매(SOD)활성증폭분별위11.9%화41.6%,과양화물매(POD)활성증폭위51.8%화77.8%,항배혈산과양화물매(APX)활성증폭위54.4%화29.1%,과양화경매(CAT)활성증폭위32.9%화38.4%,곡광감태환원매(GR)활성증폭위83.1%화85.5%.수연항양화매활성증가후회청제일부분활성양,단활성양적루적잉연조성료막지적과양화,ET8화ES8병이철(MDA)함량분별증가18.2%화50.0%,질막투성야수착MDA함량적승고이증가,증폭분별위1.25배화1.36배.종상소술,불동내려품충간활성양대사적차이시소맥품충내려성차이현저적원인지일.
The production and accumulation of reactive oxygen species (ROS) in plant can damage the structure and function of tissues under stress conditions. Meanwhile, plant can adapt to stress conditions by changing the activities of active oxygen metabo-lism enzymes to clear and alleviate the damage of ROS. In this study, Al-tolerant wheat (Triticum aestivum L.) ET8 and Al-sensitive wheat ES8 were supplied to investigate the relationship between active oxygen metabolism and Al tolerance in wheat. The results showed that the contents of active oxygen increased significantly in Al-tolerant wheat ET8 and Al-sensitive wheat ES8 with the treatments of 50 μmol·L~(-1) Al for 24 h. The O_2~(·-) in ET8 and ES8 increased by 10.5% and 20.4% respectively, and the H_2O_2 increased by 3.3% and 7.6%. The super oxide dismutase (SOD) in ET8 and ES8 increased by 11.9% and 41.6% respectively, the peroxidase (POD) increased by 51.8% and 77.8% respectively, the aacorbate peroxidase (APX) increased by 54.4% and 29.1% respectively, the catalase (CAT) increased by 32.9% and 38.4% respectively, and the glutathione reductase (GR) increased by 83.1% and 85.5% re-spectively. Although parts of the ROS could be cleared by active oxygen metabolism enzymes, the accumulation of ROS still in-duced the peroxidation of membrane lipids. The malondialdehyde (MDA) in ET8 and ES8 increased by 18.2% and 50.0% respec-tively, and the membrane permeability increased by 1.25 and 1.36 times respectively. In conclusion, the significant difference in active oxygen metabolism is one of main reasons that cause the Al-resistant difference among different Al-tolerant wheat species.