中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2010年
1期
128-131
,共4页
胡剑江%雷洪涛%侯燕鸣%王毅
鬍劍江%雷洪濤%侯燕鳴%王毅
호검강%뢰홍도%후연명%왕의
细胞迁移%活细胞工作站%细胞划痕%动态观察%小檗碱%Rg3
細胞遷移%活細胞工作站%細胞劃痕%動態觀察%小檗堿%Rg3
세포천이%활세포공작참%세포화흔%동태관찰%소벽감%Rg3
cell migration%living cell working station%cell scratch%dynamic observation%berberine%Rg3
目的 建立肿瘤细胞迁移过程的多角度、实时动态、定量评估方法.方法 建立细胞迁移观测模型,分别加入小檗碱及Rg3培养24 h,用活细胞工作站随机选取8个不同观测点,连续观测24 h.所取得的数据从迁移面积、距离、单个细胞迁移速度、单位面积细胞增殖数量等多角度进行分析.结果 该方法可以长时间观察划痕内细胞的迁移过程;小檗碱高、中剂量组(25,12.5 μmol·L~(-1))可抑制A549细胞的迁移过程(P<0.01),且迁移面积、距离、速度及细胞增殖数量4项指标的结果基本一致,人参皂苷Rg3(0.1 mmol·L~(-1))可以在各时间点(6,12,24 h)抑制细胞迁移速度(P<0.01),24 h可抑制细胞迁移面积及距离(P<0.01),但可促进细胞增殖(P<0.01).结论 该方法灵敏、简便、快速,可广泛应用于细胞迁移过程的动态观察.
目的 建立腫瘤細胞遷移過程的多角度、實時動態、定量評估方法.方法 建立細胞遷移觀測模型,分彆加入小檗堿及Rg3培養24 h,用活細胞工作站隨機選取8箇不同觀測點,連續觀測24 h.所取得的數據從遷移麵積、距離、單箇細胞遷移速度、單位麵積細胞增殖數量等多角度進行分析.結果 該方法可以長時間觀察劃痕內細胞的遷移過程;小檗堿高、中劑量組(25,12.5 μmol·L~(-1))可抑製A549細胞的遷移過程(P<0.01),且遷移麵積、距離、速度及細胞增殖數量4項指標的結果基本一緻,人參皂苷Rg3(0.1 mmol·L~(-1))可以在各時間點(6,12,24 h)抑製細胞遷移速度(P<0.01),24 h可抑製細胞遷移麵積及距離(P<0.01),但可促進細胞增殖(P<0.01).結論 該方法靈敏、簡便、快速,可廣汎應用于細胞遷移過程的動態觀察.
목적 건립종류세포천이과정적다각도、실시동태、정량평고방법.방법 건립세포천이관측모형,분별가입소벽감급Rg3배양24 h,용활세포공작참수궤선취8개불동관측점,련속관측24 h.소취득적수거종천이면적、거리、단개세포천이속도、단위면적세포증식수량등다각도진행분석.결과 해방법가이장시간관찰화흔내세포적천이과정;소벽감고、중제량조(25,12.5 μmol·L~(-1))가억제A549세포적천이과정(P<0.01),차천이면적、거리、속도급세포증식수량4항지표적결과기본일치,인삼조감Rg3(0.1 mmol·L~(-1))가이재각시간점(6,12,24 h)억제세포천이속도(P<0.01),24 h가억제세포천이면적급거리(P<0.01),단가촉진세포증식(P<0.01).결론 해방법령민、간편、쾌속,가엄범응용우세포천이과정적동태관찰.
Aim To establish a new,multi-parameter,real time and qualitative cell migration evaluation method.Methods Lung cancer cell line A549 was cultured on the glass bottom dish.After treated with different dosages of berberine or Rg3 for 24 hours,several scratching lines at the same dimension were made and observed by Living Cell Working Station.8 observation areas were selected randomly and imaged continuously for 24 hours.Transferred Area(TA),Transferred Distance(TD),Single Cell Transferred Speed(SCTS)and the Cell Division Number among defined area(CDN)were analyzed after getting sequence images.Results The focus stage and the incubation system were sufficient to keep cell proliferation and made it possible for long term observation.Berberine at 25 μmol·L~(-1) and 12.5 μmol·L~(-1) could inhibit the migration of A549(P<0.01).The analysis results of TA,TD,SCTS and CDN were basically coincident.Rg3 at 0.1 mmol·L~(-1) could inhibit SCTS and promote CDN in 6,12 and 24 h(P<0.01),while decrease both TA and TD in 24 hs.Conclusion The method is sensitive,rapid and simple to be applied in the research of tumor metastasis,wound healing and inflammatory response with real time,in-situ and multi-parameters.
