CAJ | 학술논문

背景:目前,临床上缺乏有效的预测深静脉血栓的手段.KLF2、KLF4在血栓形成前及形成过程中表达下调,有可能作为分子标记物预测诊断深静脉血栓.目的:探讨抗凝基因KLF2、KLF4预测诊断深静脉血栓的可行性.方法:从100只大鼠中随机取90只建立大鼠创伤性深静脉血栓模型,按取材时间点及是否有血栓形成分为血栓形成前组、血栓形成组和血栓消溶组,其余的10只大鼠作为正常组.在相应时间点采集各组大鼠血液,荧光实时定量PCR法检测各组大鼠血液中KLF2及KLF4 mRNA的表达.结果与结论:荧光实时定量PCR结果显示血栓形成前及血栓形成组KLF2和KLF4 mRNA表达低于正常组,而血栓消溶组血清KLF2和KLF4 mRNA水平较正常组高.提示KLF2及KLF4可能成为预测诊断深静脉血栓的分子标记物.
배경:목전,림상상결핍유효적예측심정맥혈전적수단.KLF2、KLF4재혈전형성전급형성과정중표체하조,유가능작위분자표기물예측진단심정맥혈전.목적:탐토항응기인KLF2、KLF4예측진단심정맥혈전적가행성.방법:종100지대서중수궤취90지건립대서창상성심정맥혈전모형,안취재시간점급시부유혈전형성분위혈전형성전조、혈전형성조화혈전소용조,기여적10지대서작위정상조.재상응시간점채집각조대서혈액,형광실시정량PCR법검측각조대서혈액중KLF2급KLF4 mRNA적표체.결과여결론:형광실시정량PCR결과현시혈전형성전급혈전형성조KLF2화KLF4 mRNA표체저우정상조,이혈전소용조혈청KLF2화KLF4 mRNA수평교정상조고.제시KLF2급KLF4가능성위예측진단심정맥혈전적분자표기물.
BACKGROUND:There is lack of an effective measuring means to diagnose deep venous thrombosis (DVT) in clinic.KLF2 and KLF4 are down-expressed at prethrombotic state,which may be served as predictive molecular markers to diagnose DVT.OBJECTIVE:To explore the feasibility of KLF2 and KLF4 as molecular markers to prediagnose DVT in rats.METHODS:Totally 90 rats were obtained from 100 rats to establish traumatic DVT models and divided into the prethrombotic,thrombosis crest-time and non-thrombosis groups.The remained 10 rats served as control group.Rat blood was collected at each time point,and the expressions of KLF2 and KLF4 were detected by real-time PCR.RESULTS AND CONCLUSION:The KLF2 and KLF4 mRNA expressions in the prethrombotic group and thrombosis crest-time group were lower than that of the control group.However,the KLF2 and KLF4 mRNA expressions in the non-thrombosis group was higher than that of the control group.Therefore,KLF2 and KLF4 may be candidate molecular markers for prediagnosis of DVT in rats.