中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
1期
42-44
,共3页
哮喘%血管内皮生长因子类%内皮抑素类%气管
哮喘%血管內皮生長因子類%內皮抑素類%氣管
효천%혈관내피생장인자류%내피억소류%기관
Asthma%Vascular endothelial growth factors%Endostatins%Trachea
目的 探讨哮喘豚鼠气道中血管内皮细胞生长因子(VEGF)和内皮抑素(Es)动态变化对气道重塑的作用.方法 将40只豚鼠随机分为正常对照组(C组)、生理盐水组(N组)、哮喘3 d组(A1组)、哮喘7 d组(A2组)和哮喘21 d组(A3组),8只/组.C组豚鼠不行任何处理.N组分别于1 d和8 d腹腔注射生理盐水2 ml,15 d开始用37℃生理盐水雾化吸入,1次/d,3-5min/次,共3d;A1、A2和A3组用卵蛋白(OVA)10mg、氢氧化铝20 mg制成2 ml悬液代替生理盐水作腹腔注射,用1%OVA代替生理盐水分别水雾化吸入3 d、7 d和21 d,其他方法同N组.末次激发24 h后麻醉行支气管肺泡灌洗,收集支气管肺泡灌洗液(BALF)用酶联免疫(ELISA)法测VEGF和ES浓度.用图像分析仪测定气道内周径(Pi),外周径(Pe),并计算管壁面积(WA),气道平滑肌面积(SMC-A),气道平滑肌细胞数(N),且WA、SMC-A、N均用Pi进行标准化.结果 哮喘豚鼠随病程延长,WA/Pi、SMCA/Pi、N/Pi和气道中VEGF浓度进行性升高,与C组和N组比较,哮喘各组中SMC-A/Pi、N/Pi和VEGF浓度差异有统计学意义,A2和A3组中WA/PI差异有统计学意义.哮喘豚鼠气道中VEGF浓度与WA/Pi呈正相关(r=0.51,P<0.05),VEGF/ES与WA/Pi呈正相关(r=0.78,P<0.01).结论 VEGF/ES平衡失调可能在哮喘豚鼠气道重塑起重要作用.
目的 探討哮喘豚鼠氣道中血管內皮細胞生長因子(VEGF)和內皮抑素(Es)動態變化對氣道重塑的作用.方法 將40隻豚鼠隨機分為正常對照組(C組)、生理鹽水組(N組)、哮喘3 d組(A1組)、哮喘7 d組(A2組)和哮喘21 d組(A3組),8隻/組.C組豚鼠不行任何處理.N組分彆于1 d和8 d腹腔註射生理鹽水2 ml,15 d開始用37℃生理鹽水霧化吸入,1次/d,3-5min/次,共3d;A1、A2和A3組用卵蛋白(OVA)10mg、氫氧化鋁20 mg製成2 ml懸液代替生理鹽水作腹腔註射,用1%OVA代替生理鹽水分彆水霧化吸入3 d、7 d和21 d,其他方法同N組.末次激髮24 h後痳醉行支氣管肺泡灌洗,收集支氣管肺泡灌洗液(BALF)用酶聯免疫(ELISA)法測VEGF和ES濃度.用圖像分析儀測定氣道內週徑(Pi),外週徑(Pe),併計算管壁麵積(WA),氣道平滑肌麵積(SMC-A),氣道平滑肌細胞數(N),且WA、SMC-A、N均用Pi進行標準化.結果 哮喘豚鼠隨病程延長,WA/Pi、SMCA/Pi、N/Pi和氣道中VEGF濃度進行性升高,與C組和N組比較,哮喘各組中SMC-A/Pi、N/Pi和VEGF濃度差異有統計學意義,A2和A3組中WA/PI差異有統計學意義.哮喘豚鼠氣道中VEGF濃度與WA/Pi呈正相關(r=0.51,P<0.05),VEGF/ES與WA/Pi呈正相關(r=0.78,P<0.01).結論 VEGF/ES平衡失調可能在哮喘豚鼠氣道重塑起重要作用.
목적 탐토효천돈서기도중혈관내피세포생장인자(VEGF)화내피억소(Es)동태변화대기도중소적작용.방법 장40지돈서수궤분위정상대조조(C조)、생리염수조(N조)、효천3 d조(A1조)、효천7 d조(A2조)화효천21 d조(A3조),8지/조.C조돈서불행임하처리.N조분별우1 d화8 d복강주사생리염수2 ml,15 d개시용37℃생리염수무화흡입,1차/d,3-5min/차,공3d;A1、A2화A3조용란단백(OVA)10mg、경양화려20 mg제성2 ml현액대체생리염수작복강주사,용1%OVA대체생리염수분별수무화흡입3 d、7 d화21 d,기타방법동N조.말차격발24 h후마취행지기관폐포관세,수집지기관폐포관세액(BALF)용매련면역(ELISA)법측VEGF화ES농도.용도상분석의측정기도내주경(Pi),외주경(Pe),병계산관벽면적(WA),기도평활기면적(SMC-A),기도평활기세포수(N),차WA、SMC-A、N균용Pi진행표준화.결과 효천돈서수병정연장,WA/Pi、SMCA/Pi、N/Pi화기도중VEGF농도진행성승고,여C조화N조비교,효천각조중SMC-A/Pi、N/Pi화VEGF농도차이유통계학의의,A2화A3조중WA/PI차이유통계학의의.효천돈서기도중VEGF농도여WA/Pi정정상관(r=0.51,P<0.05),VEGF/ES여WA/Pi정정상관(r=0.78,P<0.01).결론 VEGF/ES평형실조가능재효천돈서기도중소기중요작용.
Objective To investigate dynamic changes of vascular endothelial growth factor(VEGF)and endostatin(ES)in guinea.Pig of asthma and its effect on airway remodeling.Methods 40 guinea-pigs were randomly divided into normal control group(C),NS group(N),3d asthma group(A1),7d asthma group(A2)and 21d asthma group(A3),8 pigs ofeach group·Nothing was done on C Group guinea.Pigs.N group were intraperitoneal injected 2ml NS on the Ist day and the 8th day.NS inhalation started on the 15th day,1 time every day,3-5rain for each times,lasted for 3 day.Al,A2 and A3 Group were injected with 10rag ovalbumin(OVA),which made of aluminum hydroxide 20mg suspension in place of2ml NS,in place ofNS inhalation for3d,7d and 21d.At the end of24h-shot line after anesthesia BAL.Enzynle-linked immunosorbent(ELISA)was used to determine the concentration of VEGF and ES in bronchoalveolar lavnge fluid collection(BALF).Image analysis was used to determine airway circumference(Pi),outer circumference(Pe),and calculate the wall area(WA),airway smooth muscle area(SMC-A),airway smooth muscle cells(N).WA,SMC-A and N were standardized by Pi.Results With the extension of the duration of asthmatic guinea.Pigs,WA/Pi,SMC-A/Pi,N/Pi and concentration of VEGF in the airway were increased.Compared with group C and N,SMC-A/Pi,N/Pi and concentration of VEGF in asthma group were siguitlcanfly different.WA/Pi were siguificanfly different in A2 group and A3 group.The relation of the concentration of VEGF in Asthma guinea-pigs airway and WA/Pi was positive correlation.VEGF/ES and WA/Pi was highly positive correlated.Conclusions VEGF/ES inlbalance may play all important role in the airway remodeling of asthmatic guinea-pigs.
