CAJ | 학술논문

目的了解胃泌素(GAS)mRNA及其蛋自在结直肠癌组织中的表达情况,并探讨其与结直肠癌细胞凋亡指数(AI)和凋亡调控基因Fas/FasL、天冬半胱氨酸蛋白酶(caspases)的关系.方法采用巢式RT-PCR方法检测79例结直肠癌组织中GAS的mRNA表达,用TUNEL法检测细胞凋亡情况,结直肠癌组织中GAS、Fas/FasL、caspase-3、caspase-8的蛋白表达采用免疫组织化学SP法.结果结直肠癌组织中GAS mRNA与其蛋白表达呈正相关(rGAS=0.99,P<0.01).高、中分化结直肠癌组织中GAS mRNA及其蛋白的表达明显低于低分化者(χ2(高与低)=10.47、10.23,均P<0.01;χ2(中与低)=6.68、4.95,均P<0.05);GAS mRNA及其蛋白在乳头状腺癌、管状腺癌的阳性表达率明显低于黏液印戒细胞癌及未分化癌(χ2(乳与黏印)=4.80、6.22,χ2(乳与未)=5.44、8.43,χ2(管与黏印)=4.40、4.38,χ2(管与未=4.92、6.43,均P<0.05);Dukes A、B期的GAS mRNA及其蛋白阳性表达率明显低于C、D期(χ2=4.84、4.45,均P<0.05).GAS高、中表达组的细胞AI明显低于低表达组,差异有统计学意义(q(高与低)=6.71,q(中与低):4.60,均P<0.01);FasL阳性表达率在GAS低、中、高表达3组间相比,差异具统计学意义(χ2=9.35,P<0.01),其中FasL在GAS高、中表达组的阳性表达率明显高于低表达组(χ2(高与低)=6.24,χ2(中与低)=4.74,均P<0.05).结论 GAS对结直肠癌细胞凋亡的调控可能与Fas/FasL的表达失衡及其功能和信号系统的紊乱有关.GAS的检测可作为临床结直肠癌生物学行为的评估指标之一.
목적 료해위비소(GAS)mRNA급기단자재결직장암조직중적표체정황,병탐토기여결직장암세포조망지수(AI)화조망조공기인Fas/FasL、천동반광안산단백매(caspases)적관계.방법 채용소식RT-PCR방법 검측79례결직장암조직중GAS적mRNA표체,용TUNEL법검측세포조망정황,결직장암조직중GAS、Fas/FasL、caspase-3、caspase-8적단백표체채용면역조직화학SP법.결과 결직장암조직중GAS mRNA여기단백표체정정상관(rGAS=0.99,P<0.01).고、중분화결직장암조직중GAS mRNA급기단백적표체명현저우저분화자(χ2(고여저)=10.47、10.23,균P<0.01;χ2(중여저)=6.68、4.95,균P<0.05);GAS mRNA급기단백재유두상선암、관상선암적양성표체솔명현저우점액인계세포암급미분화암(χ2(유여점인)=4.80、6.22,χ2(유여미)=5.44、8.43,χ2(관여점인)=4.40、4.38,χ2(관여미=4.92、6.43,균P<0.05);Dukes A、B기적GAS mRNA급기단백양성표체솔명현저우C、D기(χ2=4.84、4.45,균P<0.05).GAS고、중표체조적세포AI명현저우저표체조,차이유통계학의의(q(고여저)=6.71,q(중여저):4.60,균P<0.01);FasL양성표체솔재GAS저、중、고표체3조간상비,차이구통계학의의(χ2=9.35,P<0.01),기중FasL재GAS고、중표체조적양성표체솔명현고우저표체조(χ2(고여저)=6.24,χ2(중여저)=4.74,균P<0.05).결론 GAS대결직장암세포조망적조공가능여Fas/FasL적표체실형급기공능화신호계통적문란유관.GAS적검측가작위림상결직장암생물학행위적평고지표지일.
Objective To examine the correlation between the mRNA and proteins expressions of gastrin (GAS), and the association of protein expression of GAS with apoptosis index (AI) and apoptosis regulation gene Fas/FasL, caspases in colorectal cancer. Methods The expressions of GAS mRNA in tumor tissues of 79 cases with colorectal cancer were detected by nested RT-PCR. Cell apoptosis was detected by molecular biology in situ apoptosis detecting technic (TUNEL). Protein expressions of GAS, Fas/FasL, and caspases were detected by immunohistochemical staining (SP method). Results The positive correlation was found between the mRNA and proteins expressions of GAS (rGAS=0.99, P<0.01). The mRNA and protein expressions of GAS in well and moderatedly differentiated cancers were significantly lower than those in poorly differentiated cancers (χ2high vs low=10.47, 10.23, P<0.01, χ2middle vs low=6.68, 4.95, P<0.05). The mRNA and protein expressions of GAS in papillary and tubular adenocarinomas were significantly lower than those in mucinous adenocarcinomas, signet-ring cell carcinoma and undifferentiated carcinoma (χ2papillary vs mucinous and sognet-ring=4.80, 6.22, χ2papillary vs undifferentiation= 5.44, 8.43, χ2tubulsr vs mucinous and signet-ring=4.40, 4.38,χ2tubular vs undifferentiation=4.92, 6.43, P<0.05, respectively). The mRNA and protein expressions of GAS in Dukes' stages A, B were significantly lower than those in Dukes stages C, D (χ2=4.84, 4.45, P<0.01 ). The AI in GAS high and moderate expression groups of colorectal cancer were significantly lower than that in low expression group (qhigh vs low=6.71, qmiddle vs low=4.60, P<0.01). The positive expression rate of FasL was significantly different among GAS high, moderate and low expression groups of colorectal cancer(χ2=9.35, P<0.01). The positive expression rate of FasL in GAS high and moderate expression groups was higher than that in low expression group (χ2high vs low=6.24, χ2moddle vs low=4.74, P<0.05). Conclusions GAS plays an important role in the regulation of cell apoptosis in colorectal carcinoma, whose mechanism may be related to the aberrant expression of Fas/FasL. GAS will he one of the indicators of the biological behavior in colorectal carcinoma.