CAJ | 학술논문

目的研究不同频率的机械振动与白细胞介素-1β(IL-1β)刺激对兔软骨细胞分解代谢基因表达的作用.方法体外培养兔软骨细胞,应用自制复合振动仪,将振动强度0.5 g,不同频率0、45、90 Hz的机械振动分别作用于不同类别的软骨细胞,实时PCR(RT-PCR)检测软骨细胞中iNOS、COX-2的含量.结果炎性反应介质IL-1β使软骨细胞中的iNOS、COX-2基因表达升高,差异有统计学意义(P<0.01).在炎性反应介质IL-1β存在的条件下,频率为45、90 Hz振动能使软骨细胞中iNOS、COX-2的表达量下降,差异有统计学意义(P<0.05).在无炎性反应介质IL-1β存在的条件下,振动对软骨细胞产生iNOS、COX-2的表达量差异无统计学意义(P>0.05).结论炎性反应介质IL-1β能加速兔软骨细胞的去分化,合适频率的机械振动能抑制IL-1β诱导的软骨细胞分解代谢基因iNOS、COX-2的表达.
목적 연구불동빈솔적궤계진동여백세포개소-1β(IL-1β)자격대토연골세포분해대사기인표체적작용.방법 체외배양토연골세포,응용자제복합진동의,장진동강도0.5 g,불동빈솔0、45、90 Hz적궤계진동분별작용우불동유별적연골세포,실시PCR(RT-PCR)검측연골세포중iNOS、COX-2적함량.결과 염성반응개질IL-1β사연골세포중적iNOS、COX-2기인표체승고,차이유통계학의의(P<0.01).재염성반응개질IL-1β존재적조건하,빈솔위45、90 Hz진동능사연골세포중iNOS、COX-2적표체량하강,차이유통계학의의(P<0.05).재무염성반응개질IL-1β존재적조건하,진동대연골세포산생iNOS、COX-2적표체량차이무통계학의의(P>0.05).결론 염성반응개질IL-1β능가속토연골세포적거분화,합괄빈솔적궤계진동능억제IL-1β유도적연골세포분해대사기인iNOS、COX-2적표체.
Objective To investigate the effects of mechanical vibration frequency and IL-1 (interleukin-1) on expressions of catabolism genes in cultured rabbit chondrocytes. Methods Chondrocytes were obtained from the joints of a freshly killed 2-month-old rabbit. Six-well plates, half of which contained IL-1β, were used to culture samples. The well plates were vibrated at the frequency and amplitude 0/45/90 Hz and 0. 5 nm, respectively. After culture and mechanical stimulation, the levels of iNOS and COX-2 in the samples were measured by RT-PCR. Results IL-1β promoted expressions of the iNOS and COX-2 genes in the rabbit chondrocytes ( P < 0. 01). In the presence of IL-1β, mechanical vibrations of 45 and 90 HZ inhibited expression levels of iNOS and COX-2 mRNA, with a significant difference ( P < 0. 05). However, in the absence of IL-1β, mechanical vibrations of 45 and 90HZ made no significant impact on expressions of iNOS and COX-2 ( P > 0. 05). Conclusion Mechanical vibration may play an important role in curbing expressions of iNOS and COX-2 genes in the presence of IL-1.